CAJ | 학술논문

为观察醉鱼草苷Ⅳ对 H22肝癌小鼠的体内抑瘤作用,取 H22肝癌细胞接种于小鼠右腋皮下,H22肝癌小鼠随机分为5组：模型组、醉鱼草苷Ⅳ高、中、低(1．00、0．50、0．25 mg/kg)3个剂量组和阳性药(CTX,20．0 mg/kg)对照组.每组10只,治疗期间记录各组小鼠的体重变化并观察小鼠生存状态,连续腹腔注射10 d后,于第11天观测抑瘤率、脾腺指数、胸腺指数,同时进行血清中 SOD、MDA、GGT 和 AKP 含量检测.结果表明：与模型组比较,醉鱼草苷Ⅳ高、中剂量组对小鼠 H22移植性肝癌均有显著的抑瘤作用(P＜0．01),抑瘤率分别为56．96％和50．63％；与模型组相比,醉鱼草苷Ⅳ高剂量组小鼠血清SOD活性增加(P＜0．05),醉鱼草苷Ⅳ各剂量组血清MDA、GGT和AKP水平均降低(P＜0．01)；除 SOD活性之外,环磷酰胺组与模型组比较无显著差异.说明醉鱼草苷Ⅳ在体内具有抗肝癌作用,其机理可能和机体的抗氧化能力有关,有待于进一步研究。
위관찰취어초감Ⅳ대 H22간암소서적체내억류작용,취 H22간암세포접충우소서우액피하,H22간암소서수궤분위5조：모형조、취어초감Ⅳ고、중、저(1．00、0．50、0．25 mg/kg)3개제량조화양성약(CTX,20．0 mg/kg)대조조.매조10지,치료기간기록각조소서적체중변화병관찰소서생존상태,련속복강주사10 d후,우제11천관측억류솔、비선지수、흉선지수,동시진행혈청중 SOD、MDA、GGT 화 AKP 함량검측.결과표명：여모형조비교,취어초감Ⅳ고、중제량조대소서 H22이식성간암균유현저적억류작용(P＜0．01),억류솔분별위56．96％화50．63％；여모형조상비,취어초감Ⅳ고제량조소서혈청SOD활성증가(P＜0．05),취어초감Ⅳ각제량조혈청MDA、GGT화AKP수평균강저(P＜0．01)；제 SOD활성지외,배린선알조여모형조비교무현저차이.설명취어초감Ⅳ재체내구유항간암작용,기궤리가능화궤체적항양화능력유관,유대우진일보연구。
To observe the inhibitory effect of buddlej asaponinⅣon tumor growth on murine transplanted hepatocarci-noma 22 (H22)invivo,tumor inhibitory activities were tested in experimental tumor H22 mice.The tumor bearing mice were randomly divided into control group,CTX group (CTX,20.0 mg/kg)and buddlejasaponin Ⅳ treatment groups (1.00,0.50,0.25 mg/kg).The body weight and survival of mice in all groups were recorded.After exposing to medications for 10 days,all the mice were killed on second day,the tumors were taken out and weighted with tumor inhibition rate,and the indexes of spleen and thymus were checked,and the SOD activity,MDA,GGT and AKP levels in serum in each group were detected.The results showed that the high and middle dosage buddlej asaponinⅣtreated groups could inhibit H22 growth compared with the control group (P<0.01),the inhibitory rate was 56.96%, 50.63% respectively.In the high dosage buddlejasaponinⅣtreated groups,the activity of serum SOD increased (P<0.05),and in all the buddlejasaponinⅣtreated groups,serum MDA contents decreased (P<0.01).Compared with the control group,in all the buddlej asaponinⅣtreated groups,serum GGT,AKP content compared were highly sig-nificant differences (P<0.01),while the CTX group showed no significant difference.BuddlejasaponinⅣcan inhibit hepatocarcinoma growth.The mechanism may regulate the body antioxidant capacity,and needs further study.