CAJ | 학술논문

以祁连圆柏和圆柏幼苗为材料，研究不同处理时间下低温胁迫对圆柏属植物叶片亚细胞抗氧化酶活性的影响，探讨其在圆柏属植物叶片中的亚细胞定位。结果表明：低温胁迫下，丙二醛(MDA)含量和抗氧化酶活性随时间变化均呈先升后降趋势，祁连圆柏中抗氧化酶的种类比圆柏的多且活性强，而 MDA 含量低于圆柏，表明祁连圆柏在低温胁迫下具有更广泛的适应性。此外，两种圆柏植物叶片超氧化物歧化酶(SOD)和抗坏血酸过氧化物酶(APX)定位为叶绿体＞细胞溶质＞线粒体，过氧化氢酶(CAT)定位为线粒体＞叶绿体＞细胞溶质，谷胱甘肽还原酶(GR)定位为线粒体＞细胞溶质＞叶绿体，祁连圆柏过氧化物酶(POD)定位为细胞溶质＞叶绿体＞线粒体，圆柏POD定位为细胞溶质＞线粒体＞叶绿体，且抗氧化酶SOD、APX和 GR在亚细胞中分布差异达到极显著，这说明抗氧化酶在其中一种亚细胞中发挥主要作用，为克隆亚细胞组分中的抗氧化酶基因提供了理论依据。
이기련원백화원백유묘위재료，연구불동처리시간하저온협박대원백속식물협편아세포항양화매활성적영향，탐토기재원백속식물협편중적아세포정위。결과표명：저온협박하，병이철(MDA)함량화항양화매활성수시간변화균정선승후강추세，기련원백중항양화매적충류비원백적다차활성강，이 MDA 함량저우원백，표명기련원백재저온협박하구유경엄범적괄응성。차외，량충원백식물협편초양화물기화매(SOD)화항배혈산과양화물매(APX)정위위협록체＞세포용질＞선립체，과양화경매(CAT)정위위선립체＞협록체＞세포용질，곡광감태환원매(GR)정위위선립체＞세포용질＞협록체，기련원백과양화물매(POD)정위위세포용질＞협록체＞선립체，원백POD정위위세포용질＞선립체＞협록체，차항양화매SOD、APX화 GR재아세포중분포차이체도겁현저，저설명항양화매재기중일충아세포중발휘주요작용，위극륭아세포조분중적항양화매기인제공료이론의거。
Seedlings of Sabina przewalskii and S.chinensis were used to study the effects of low temperature stress on antioxidant enzymes activities and subcellular distribution in the leaves of Sabina.The results showed that:under low temperature stress,the contents of malonaldehyde (MDA)and antioxidant enzymes activities increased firstly and then decreased,and compared with Sabina chinensis,the more species and higher activities of antioxidant en-zymes were found in S.przewalskii,but MDA contents of S.przewalskii were lower than S.chinensis,it suggested that S.przewalskii showed a prevailing strategy than S.chinensis in order to develop the tolerance to freezing.Mo-reover,the localization of superoxide dismutase(SOD)and ascorbate peroxidase(APX)in the leaves of two Sabina species was chloroplast>cytosol>mitochondria,the localization of catalase(CAT )was mitochondria>chloroplast>cytosol,the localization of glutathione reductase(GR)was mitochondria>cytosol>chloroplast,the localization of per-oxidase(POD)in S.przewalskii was cytosol>chloroplast>mitochondria,the localization of POD in S.chinensis was cytosol>mitochondria>chloroplast,moreover subcellular distribution of antioxidant enzymes (SOD,APX,GR) in organelles were different significantly,this showed that antioxidant enzymes played a major role in one of organ-elles,which provided theoretical basis for cloning of antioxidant enzymes gene in subcellular fractions.