CAJ | 학술논문

MADS-box基因参与植物多种发育过程，尤其在花发育过程中发挥着重要的调控作用。该研究以玉米不育系C48-2及保持系N48-2为材料，采用RT-PCR克隆mads3基因。序列分析发现，在不育系C48-2中该基因编码区发生单碱基突变，导致其推定的蛋白质中5个氨基酸序列发生改变。荧光定量PCR显示，相对于保持系，花粉母细胞时期和四分体时期mads 3基因在C48-2下调表达，而单核期和双核期上调表达。这些结果为进一步了解玉米C型细胞质雄性不育的发生机制提供参考。
MADS-box기인삼여식물다충발육과정，우기재화발육과정중발휘착중요적조공작용。해연구이옥미불육계C48-2급보지계N48-2위재료，채용RT-PCR극륭mads3기인。서렬분석발현，재불육계C48-2중해기인편마구발생단감기돌변，도치기추정적단백질중5개안기산서렬발생개변。형광정량PCR현시，상대우보지계，화분모세포시기화사분체시기mads 3기인재C48-2하조표체，이단핵기화쌍핵기상조표체。저사결과위진일보료해옥미C형세포질웅성불육적발생궤제제공삼고。
MADS-box genes involve in various developmental processes in plant,and play an important role in the reg-ulation of floral development.In this paper,mads3,a member of MADS box gene family was cloned by RT-PCR in maize.Sequence analysis showed that 8 sits single-base substitution mutations in CDS region were found in CMS-C line in comparison to maintainer line,which may result in 5 individual amino acides change.Furthermore,real-time qPCR analysis showed that mads3 was significantly down-regulated in CMS-C line at tetrad stage,and was signifi-cantly up-regulated at uninucleate stage.These results laid the foundation for further studying the relationship be-tween MADS-box genes and CMS-C in maize.