CAJ | 학술논문

目的：初步探讨 As2 O3/CD133抗体双面涂层药物洗脱支架在动物体内的治疗作用。方法：60只中华小型猪非高脂饮食喂养4周，随机分为球囊损伤组、假手术组和双面涂层支架组，造模后继续喂养4周，行冠状动脉光学相干断层成像（OCT）后处死动物，取心肌组织行免疫组化检测。结果：OCT 图像显示，球囊损伤组冠状动脉斑块明显扩大，内膜增厚，纤维帽厚度明显增加；与球囊损伤组相比，双面涂层支架组斑块明显缩小，内膜增厚不明显，纤维帽厚度无明显增加；假手术组冠状动脉基本正常。双面涂层支架组冠状动脉的内膜/中膜面积比和厚度比低于球囊损伤组，但仍高于假手术组（P＜0．05）。免疫组化结果表明，球囊损伤组心肌 Bax 和 Bcl-22的表达明显高于假手术组，双面涂层支架组 Bax 和 Bcl-22的表达明显低于球囊损伤组（P 均＜0．01），而与假手术组无明显差异。结论：As2 O3/CD133抗体双面药物涂层支架能显著抑制冠状动脉内膜增殖，抑制心肌凋亡。
목적：초보탐토 As2 O3/CD133항체쌍면도층약물세탈지가재동물체내적치료작용。방법：60지중화소형저비고지음식위양4주，수궤분위구낭손상조、가수술조화쌍면도층지가조，조모후계속위양4주，행관상동맥광학상간단층성상（OCT）후처사동물，취심기조직행면역조화검측。결과：OCT 도상현시，구낭손상조관상동맥반괴명현확대，내막증후，섬유모후도명현증가；여구낭손상조상비，쌍면도층지가조반괴명현축소，내막증후불명현，섬유모후도무명현증가；가수술조관상동맥기본정상。쌍면도층지가조관상동맥적내막/중막면적비화후도비저우구낭손상조，단잉고우가수술조（P＜0．05）。면역조화결과표명，구낭손상조심기 Bax 화 Bcl-22적표체명현고우가수술조，쌍면도층지가조 Bax 화 Bcl-22적표체명현저우구낭손상조（P 균＜0．01），이여가수술조무명현차이。결론：As2 O3/CD133항체쌍면약물도층지가능현저억제관상동맥내막증식，억제심기조망。
Objective:To investigate the effect of a double coated drug-eluting stent of As2O3 and CD133 antibody. Methods:A total of 60 China miniature pigs were feeded with non-fat diet for 4 weeks,then randomly divided into 3 groups:ballon injury group,sham group and stent implantation group.After relative treatment,pigs were raised for another 4 weeks.All animals were sacrificed after optical coherence tomography (OCT)examination and cardiac tissue was subjected to immunohistology analysis. Resuits:It was showed by OCT that coronary plaque enlarged,and endothelium and fibrous cap thickened in balloon injury group,but vascular lesions in stent implantation group were significantly reduced.The coronary intima / media thickness ratio and area ratio in stent implantation group was lower than those in ballon injury group,but higher than those in sham group (P <0.05 ).The protein expression of Bax and Bcl-22 in ballon injury group was significantly higher than those in sham group and stent implantation group (P <0.01).But there were no significant differences between sham group and stent implantation group. Conciusion:As2 O3/CD133 antibody double coated drug-eluting stent significantly inhibits intimal proliferation and reduces myocardial apoptosis.