CAJ | 학술논문

目的：探讨诱导 P19细胞向心肌样细胞分化前后 gp91-phox 水平的变化。方法：P19细胞经0．9％二甲基亚砜（DMSO）于细菌培养皿中悬浮诱导培养4 d，待细胞聚集体形成，吸取聚集体接种于组织培养皿，贴壁培养至第13天。行 Western blot 检测肌钙蛋白 I（cTnI），以鉴定心肌样细胞的分化，并检测 P19细胞向心肌样细胞分化前后细胞中 gp91-phox 蛋白水平。结果：（1）经0．9％ DMSO 诱导分化，P19细胞于第7天开始表达 cTnI，随后 cTnI 表达明显增高并趋于稳定；（2）P19细胞分化为心肌样细胞后细胞内 gp91-phox 蛋白水平较分化前高（P ＜0．05）。结论：P19细胞向心肌样细胞分化后 gp91-phox 表达上调，氧化应激水平增加。
목적：탐토유도 P19세포향심기양세포분화전후 gp91-phox 수평적변화。방법：P19세포경0．9％이갑기아풍（DMSO）우세균배양명중현부유도배양4 d，대세포취집체형성，흡취취집체접충우조직배양명，첩벽배양지제13천。행 Western blot 검측기개단백 I（cTnI），이감정심기양세포적분화，병검측 P19세포향심기양세포분화전후세포중 gp91-phox 단백수평。결과：（1）경0．9％ DMSO 유도분화，P19세포우제7천개시표체 cTnI，수후 cTnI 표체명현증고병추우은정；（2）P19세포분화위심기양세포후세포내 gp91-phox 단백수평교분화전고（P ＜0．05）。결론：P19세포향심기양세포분화후 gp91-phox 표체상조，양화응격수평증가。
Objective:To investigate the changes of gp91-phox expression before and after the differentiation of P19 cells to cardiomyocyte-like cells. Methods:P19 cells were cultured with 0.9% dimethyl sulfoxide(DMSO)in suspension for 4 days to form aggregation.Then the aggregates were transferred to tissue culture dishes for further cultivation up to the 13th day.Cardiac troponin I (cTnI )was detected by Western blot to identify cell differentiation,then the expression levels of gp91-phox were detected by Western blot before and after the differentiation. Resuits:(1)P19 cells induced by 0.9% DMSO were cTnI-positive at the 7th day,afterwards the expression of cTnI was gradually increased and maintained at a stable level.(2)The expression of gp91-phox in differentiated P19 cells was higher than that in undifferentiated cells (P <0.05). Conciusion:The expression of gp91-phox was up-regulated after the differentiation of P19 cells to cardiomyocyte-like cells indicating the increased oxidative stress level during this process.