神经损伤与功能重建
神經損傷與功能重建
신경손상여공능중건
NEURAL INJURY AND FUNCTIONAL RECONSTRUCTION
2014年
5期
376-378
,共3页
倪厚杰%刘娜%李新华%唐洲平
倪厚傑%劉娜%李新華%唐洲平
예후걸%류나%리신화%당주평
脑出血%神经功能缺损%免疫组织化学%神经干细胞%血管内皮生长因子
腦齣血%神經功能缺損%免疫組織化學%神經榦細胞%血管內皮生長因子
뇌출혈%신경공능결손%면역조직화학%신경간세포%혈관내피생장인자
intracerebral hemorrhage%neurologic impairment%immunohistochemistry%neural stem cells%vascu-lar endothelial growth factor
目的：观察外源性神经生长因子（NGF）治疗大鼠脑出血的疗效。方法：48只SD大鼠随机分成NGF组24只、脑出血组12只和假手术组12只。Ⅶ型胶原酶尾状核注射制备大鼠脑出血模型；NGF组给予NGF血肿内注射，脑出血组注射等量生理盐水，假手术组只进针。分别于术后1、3及7d对3组大鼠进行神经功能评分，免疫组化法检测血肿灶周Nestin及血管内皮生长因子（VEGF）的阳性细胞数。结果：造模后7 d， NGF组神经功能缺损评分降低，低于NGF组1 d及3 d时的评分（＜0.05），且低于脑出血组7 d时的评分（＜0.05）。各时间点NGF组Nestin及VEGF阳性细胞数均显著高于假手术组（＜0.01），高于脑出血组（＜0.05）；NGF组7 d时Nestin及VEGF阳性细胞数高于同组1 d及3 d时（＜0.05）。结论：外源性NGF有助于促进脑出血大鼠的神经功能恢复，其机制可能与激活脑出血大鼠的内源性神经干细胞及促进脑组织分泌VEGF有关。
目的：觀察外源性神經生長因子（NGF）治療大鼠腦齣血的療效。方法：48隻SD大鼠隨機分成NGF組24隻、腦齣血組12隻和假手術組12隻。Ⅶ型膠原酶尾狀覈註射製備大鼠腦齣血模型；NGF組給予NGF血腫內註射，腦齣血組註射等量生理鹽水，假手術組隻進針。分彆于術後1、3及7d對3組大鼠進行神經功能評分，免疫組化法檢測血腫竈週Nestin及血管內皮生長因子（VEGF）的暘性細胞數。結果：造模後7 d， NGF組神經功能缺損評分降低，低于NGF組1 d及3 d時的評分（＜0.05），且低于腦齣血組7 d時的評分（＜0.05）。各時間點NGF組Nestin及VEGF暘性細胞數均顯著高于假手術組（＜0.01），高于腦齣血組（＜0.05）；NGF組7 d時Nestin及VEGF暘性細胞數高于同組1 d及3 d時（＜0.05）。結論：外源性NGF有助于促進腦齣血大鼠的神經功能恢複，其機製可能與激活腦齣血大鼠的內源性神經榦細胞及促進腦組織分泌VEGF有關。
목적：관찰외원성신경생장인자（NGF）치료대서뇌출혈적료효。방법：48지SD대서수궤분성NGF조24지、뇌출혈조12지화가수술조12지。Ⅶ형효원매미상핵주사제비대서뇌출혈모형；NGF조급여NGF혈종내주사，뇌출혈조주사등량생리염수，가수술조지진침。분별우술후1、3급7d대3조대서진행신경공능평분，면역조화법검측혈종조주Nestin급혈관내피생장인자（VEGF）적양성세포수。결과：조모후7 d， NGF조신경공능결손평분강저，저우NGF조1 d급3 d시적평분（＜0.05），차저우뇌출혈조7 d시적평분（＜0.05）。각시간점NGF조Nestin급VEGF양성세포수균현저고우가수술조（＜0.01），고우뇌출혈조（＜0.05）；NGF조7 d시Nestin급VEGF양성세포수고우동조1 d급3 d시（＜0.05）。결론：외원성NGF유조우촉진뇌출혈대서적신경공능회복，기궤제가능여격활뇌출혈대서적내원성신경간세포급촉진뇌조직분비VEGF유관。
Objective:To observe the therapeutic effect of exogenous nerve growth factor (NGF) on rats after intracerebral hemorrhage(ICH). Methods:Forty-eight SD rats were randomly divided into NGF group (n=24), ICH group (n=12) and sham group (n=12). ICH models were made by injecting Ⅶ collagenase into caudate nucleus of rats. Rats in NGF group were treated with NGF whereas those in ICH group received normal saline. The neurolog-ical function was evaluated, and the Nestin and vascular endothelial growth factor (VEGF) positive cells located perihematoma were detected by immunohistochemistry at 1, 3 and 7 days after operation respectively. Results:The neurological severity score at 7 d after operation was lower than those at 1 and 3 days after operation in NGF group ( <0.05). The neurological severity score of NGF group was lower than that of ICH group at 7 days after operation ( <0.05). The numbers of VEGF and Nestin positive cells in NGF group were significantly higher than those in sham group ( <0.01) and those in ICH group ( <0.05) at 1, 3, 7 days after operation. The numbers of VEGF and Nestin positive cells at 7 days after operation was greater than those at 1 and 3 days after operation in NGF group ( <0.05). Conclusion:Exogenous NGF contributes to the improvement of neural function recovery of rats after ICH, which may be mediated by activation of endogenous neural stem cells and increase of VEGF expression.
