CAJ | 학술논문

为探究维生素C对镉导致鲫（ Carassius auratus）红细胞微核的干预作用，用0.5 mg/L的镉溶液单一染毒作对照组，设置0.5 mg/L的镉溶液分别添加1、2、3 g/L的维生素C（ VC）三组作为实验组。在染毒后的第5、10、15、20天分别取鲫尾部血液制作血涂片，镜检并统计红细胞微核率。结果显示：0.5 mg/L的镉溶液单一染毒对照组，镉诱发鲫红细胞产生微核率与时间呈现线性关系，回归函数为： y＝0.1587 x＋2.5167（ R2＝0.9357）；实验组随着VC浓度增加和处理时间的延长，微核率降低，其中以0.5 mg/L Cd2＋＋3 g/L VC组处理20 d的微核率最低，微核率为仅为0.26‰，与处理20 d的对照组相比，微核抑制率达到95.62％。 T检验分析表明， VC对镉导致鲫鱼红细胞微核干预作用极显著（ t＞t0.01），干预作用在试验中呈现出随VC浓度增加干预作用增强的趋势。实验表明， VC对镉导致鲫红细胞微核率升高的现象有明显的干预作用。
위탐구유생소C대력도치즉（ Carassius auratus）홍세포미핵적간예작용，용0.5 mg/L적력용액단일염독작대조조，설치0.5 mg/L적력용액분별첨가1、2、3 g/L적유생소C（ VC）삼조작위실험조。재염독후적제5、10、15、20천분별취즉미부혈액제작혈도편，경검병통계홍세포미핵솔。결과현시：0.5 mg/L적력용액단일염독대조조，력유발즉홍세포산생미핵솔여시간정현선성관계，회귀함수위： y＝0.1587 x＋2.5167（ R2＝0.9357）；실험조수착VC농도증가화처리시간적연장，미핵솔강저，기중이0.5 mg/L Cd2＋＋3 g/L VC조처리20 d적미핵솔최저，미핵솔위부위0.26‰，여처리20 d적대조조상비，미핵억제솔체도95.62％。 T검험분석표명， VC대력도치즉어홍세포미핵간예작용겁현저（ t＞t0.01），간예작용재시험중정현출수VC농도증가간예작용증강적추세。실험표명， VC대력도치즉홍세포미핵솔승고적현상유명현적간예작용。
To investigate the effect of vitamin C intervention on cadmium induced erythrocyte micronucleus of Carassius au-ratus, with the 0.5 mg/L cadmium solution as the control group , the 0.5 mg/L cadmium solution separately added 1 g, 2 g, 3 g/L of vitamin C(VC) were as experimental group.Firstly, C.auratus were separately exposure 5, 10, 15, 20 d. Then, blood smears were taken from crucian carp tail blood , microscopic examination , and the erythrocyte micronucleus rate was measured .The results showed that the erythrocyte micronucleus rate induced by cadmium increased linearly with time in the control group, y=0.158 7 X+2.516 7 (R2 =0.935 7).However, in the experimental group, the erythro-cyte micronucleus rate decreased with vitamin C concentration increasing and treatment time .The micronucleus rate was lowest for the experimental group with 3 g/L vitamin C and treating 20 d, and only 0.26%; contrast to the control group treating 20 d, control rate of micronucleus was 95.62%.The T-test results showed that the effect of vitamin C intervention on cadmium induced erythrocyte micronucleus rate was significant ( t>t0.01 ) , the effect increased with vitamin C concen-tration increasing .