CAJ | 학술논문

为探究亚硝态氮对于草鱼离体肝细胞抗氧化系统的影响，将分离得到的离体草鱼肝细胞在含0（空白），1，10和100 mg/L （浓度以氮计）的亚硝酸钠的M199生长培养基中，分别培养6、12、24、48和96 h后，检测肝细胞中超氧化物歧化酶（ SOD ）、过氧化氢酶（ CAT ）、γ-谷氨酰半胱氨酸合成酶（γ-GCS ）和谷胱甘肽转移酶（GST）的活性，谷胱甘肽（GSH）水平以及总抗氧化能力（TAC）。结果显示：高浓度的亚硝酸钠会引起肝细胞内的SOD、 CAT、 GST、γ-GCS活性显著升高；除100 mg/L实验组外，各实验组的 GSH水平均显著高于对照组，而TAC则随着亚硝酸钠浓度的升高，表现为先升高后下降的规律，且当亚硝酸钠浓度为10 mg/L时， TAC达最高水平。结果表明，较低剂量的亚硝态氮急性暴露可激活肝细胞抗氧化系统并促进其提高抗氧化能力，而高剂量亚硝态氮（100 mg/L）则显著降低了肝细胞的抗氧化能力。
위탐구아초태담대우초어리체간세포항양화계통적영향，장분리득도적리체초어간세포재함0（공백），1，10화100 mg/L （농도이담계）적아초산납적M199생장배양기중，분별배양6、12、24、48화96 h후，검측간세포중초양화물기화매（ SOD ）、과양화경매（ CAT ）、γ-곡안선반광안산합성매（γ-GCS ）화곡광감태전이매（GST）적활성，곡광감태（GSH）수평이급총항양화능력（TAC）。결과현시：고농도적아초산납회인기간세포내적SOD、 CAT、 GST、γ-GCS활성현저승고；제100 mg/L실험조외，각실험조적 GSH수평균현저고우대조조，이TAC칙수착아초산납농도적승고，표현위선승고후하강적규률，차당아초산납농도위10 mg/L시， TAC체최고수평。결과표명，교저제량적아초태담급성폭로가격활간세포항양화계통병촉진기제고항양화능력，이고제량아초태담（100 mg/L）칙현저강저료간세포적항양화능력。
To investigate the effects of nitrite on the anti-oxidative system of grass carp ( Ctenopharyngodon idle lus) isola-ted hepatocyte, the hepatocytes were exposed to four experimental groups (0, 1, 10 and 100 mg nitrite per liter).The activities of superoxide dismutase (SOD), catalase (CAT), γ-gutamylcysteine synthetase (γ-GCS) and glutathione S-transferase (GST), glutathione (GSH) content,and the total antioxidant capacity (TAC) were assayed at 6, 12, 24, 48 and 96 h, respectively after exposure .The results showed that the activities of SOD、 CAT、 GST andγ-GCS increased significantly after exposure in higher dose groups .The contents of GSH in hepatocytes exposed to nitrite were significantly higher than that in control group except for the 100 mg/L group.Nitrite exposure exerted deleterious effect on the TAC of hepatocytes and its activity increased initial and then declined .The TAC exposed to 10 mg/L nitrite was significantly high-er than that of other groups .In short , acute exposure of low dose nitrite could activate the antioxidant system of hepato-cytes and promote the antioxidant capacity , but high dose nitrite might depress the antioxidant capacity significantly .