CAJ | 학술논문

为探讨饲喂蚕豆（ Vicia faba）对草鱼（ Ctenopharyngodon idellus）肠道菌群的影响，采用PCR-DGGE技术比较了饲喂蚕豆的草鱼（脆肉鲩组）及饲喂配合饲料的草鱼（普通草鱼组）肠道微生物菌群的异同。结果显示， DGGE图谱上出现了20条明显条带，表明脆肉鲩组及普通草鱼组肠道中均存在大量细菌群落。对这20条条带测序后，获得了其中17条条带的序列，这17条条带中有9条是尚未被培养的细菌。经分析发现，这17条条带分属于变形菌门（ Proteobacteria ）、放线菌门（ Actinobacteria ）、厚壁菌门（ Firmicutes ）、拟杆菌门（ Bacteroidetes ）及未分类的细菌，其中变形菌门为两组肠道的优势菌。实验还发现，饲喂蚕豆对肠内容物菌群的影响大于对肠壁菌群的影响。结果表明，饲喂蚕豆不改变草鱼肠道菌群的种类，但对肠道菌群的相对丰度有一定影响。
위탐토사위잠두（ Vicia faba）대초어（ Ctenopharyngodon idellus）장도균군적영향，채용PCR-DGGE기술비교료사위잠두적초어（취육혼조）급사위배합사료적초어（보통초어조）장도미생물균군적이동。결과현시， DGGE도보상출현료20조명현조대，표명취육혼조급보통초어조장도중균존재대량세균군락。대저20조조대측서후，획득료기중17조조대적서렬，저17조조대중유9조시상미피배양적세균。경분석발현，저17조조대분속우변형균문（ Proteobacteria ）、방선균문（ Actinobacteria ）、후벽균문（ Firmicutes ）、의간균문（ Bacteroidetes ）급미분류적세균，기중변형균문위량조장도적우세균。실험환발현，사위잠두대장내용물균군적영향대우대장벽균군적영향。결과표명，사위잠두불개변초어장도균군적충류，단대장도균군적상대봉도유일정영향。
To evaluate the effect of faba bean on intestinal flora , the intestinal microbiota of crisp grass carp group ( grass carp fed with faba bean ) and grass carp group ( grass carp fed with formula feed ) were investigated using 16S rDNA PCR denaturing gradient gel electrophoresis (PCR-DGGE) technology.Twenty DGGE bands appearing in DGGE fingerprint re-vealed that there were numerous intestinal microbiota in the two groups .Seventeen DGGE bands were successfully se-quenced.However, nine of these bands were classified as unculturable according to the phylogenetic analysis .The domi-nant bacteria in the fore-gut and mid-gut of the two groups all belonged to Proteobacteria , and other bacteria identified be-longed to actinobacteria, firmicutes, bacteroidetes and unclassified-bacteria.The result also showed that the influence caused by faba bean on intestinal microbiota was more remarkable in intestinal content than in intestinal wall .These results suggested that feeding faba bean did not change the microbial diversity of grass carp intestine , but affected the relative a-bundance of some bacteria .