淡水渔业
淡水漁業
담수어업
FRESHWATER FISHERIES
2014年
5期
9-14
,共6页
张丽娟%祝斐%尹绍武%张亚萍%侯新远%胡亚丽
張麗娟%祝斐%尹紹武%張亞萍%侯新遠%鬍亞麗
장려연%축비%윤소무%장아평%후신원%호아려
河川沙塘鳢(Odontobutis potamophila)%鸭绿沙塘鳢(O. yaluensis)%杂交子代%微卫星%遗传关系
河川沙塘鱧(Odontobutis potamophila)%鴨綠沙塘鱧(O. yaluensis)%雜交子代%微衛星%遺傳關繫
하천사당례(Odontobutis potamophila)%압록사당례(O. yaluensis)%잡교자대%미위성%유전관계
Odontobutis potamophila%O.yaluensis%hybrid progeny%microsatellite%hereditary relationship
使用15对微卫星引物对河川沙塘鳢（ Odontobutis potamophila，♀）、鸭绿沙塘鳢（ O.yaluensis，♂）及其河川沙塘鳢（♀）×鸭绿沙塘鳢（♂）杂交子代3个群体进行扩增，研究3个群体的分子遗传机制，结果表明：（1）15对微卫星引物中OP215 a和OP110 a在鸭绿沙塘鳢中呈现单态性，其余的13对在3个群体中均能良好扩增；OP10a、 OP23a和OP46a可鉴别河川沙塘鳢、鸭绿沙塘鳢及杂交子代3个群体。（2）3个群体的平均多态信息（ PIC）含量分别为0.6450、0.5375、0.5183；平均有效等位基因数（ Ne）分别为4.2977、3.6188、2.7793；平均观测杂合度（Ho）分别为0.6334、0.5920、0.6290。（3）双亲遗传分化显著，等位基因均来自亲本群体，基本符合孟德尔遗传定律，推断杂交子代为两性融合生殖，是真正意义上的杂交种。（4）杂交子代与河川沙塘鳢和鸭绿沙塘鳢的遗传距离分别为1.0036、0.8361，前者大于后者，表明杂交子代与两亲本的遗传差异是不对等的，而是偏向父本一方，系统进化树也支持这一观点。
使用15對微衛星引物對河川沙塘鱧（ Odontobutis potamophila，♀）、鴨綠沙塘鱧（ O.yaluensis，♂）及其河川沙塘鱧（♀）×鴨綠沙塘鱧（♂）雜交子代3箇群體進行擴增，研究3箇群體的分子遺傳機製，結果錶明：（1）15對微衛星引物中OP215 a和OP110 a在鴨綠沙塘鱧中呈現單態性，其餘的13對在3箇群體中均能良好擴增；OP10a、 OP23a和OP46a可鑒彆河川沙塘鱧、鴨綠沙塘鱧及雜交子代3箇群體。（2）3箇群體的平均多態信息（ PIC）含量分彆為0.6450、0.5375、0.5183；平均有效等位基因數（ Ne）分彆為4.2977、3.6188、2.7793；平均觀測雜閤度（Ho）分彆為0.6334、0.5920、0.6290。（3）雙親遺傳分化顯著，等位基因均來自親本群體，基本符閤孟德爾遺傳定律，推斷雜交子代為兩性融閤生殖，是真正意義上的雜交種。（4）雜交子代與河川沙塘鱧和鴨綠沙塘鱧的遺傳距離分彆為1.0036、0.8361，前者大于後者，錶明雜交子代與兩親本的遺傳差異是不對等的，而是偏嚮父本一方，繫統進化樹也支持這一觀點。
사용15대미위성인물대하천사당례（ Odontobutis potamophila，♀）、압록사당례（ O.yaluensis，♂）급기하천사당례（♀）×압록사당례（♂）잡교자대3개군체진행확증，연구3개군체적분자유전궤제，결과표명：（1）15대미위성인물중OP215 a화OP110 a재압록사당례중정현단태성，기여적13대재3개군체중균능량호확증；OP10a、 OP23a화OP46a가감별하천사당례、압록사당례급잡교자대3개군체。（2）3개군체적평균다태신식（ PIC）함량분별위0.6450、0.5375、0.5183；평균유효등위기인수（ Ne）분별위4.2977、3.6188、2.7793；평균관측잡합도（Ho）분별위0.6334、0.5920、0.6290。（3）쌍친유전분화현저，등위기인균래자친본군체，기본부합맹덕이유전정률，추단잡교자대위량성융합생식，시진정의의상적잡교충。（4）잡교자대여하천사당례화압록사당례적유전거리분별위1.0036、0.8361，전자대우후자，표명잡교자대여량친본적유전차이시불대등적，이시편향부본일방，계통진화수야지지저일관점。
Genetic relationship of Odontobutis potamophila (♀) , O.yaluensis (♂) and their hybrid progeny were ana-lyzed using fifteen microsatellite loci to amplify the genomic DNA .The result showed: (1) thirteen pairs of primers gave clear bands in 3 poputionlations; OP215a and OP110 were showed monomorphism in O.yaluensis; (2)The average of pol-ymorphism information contents ( PIC) values in three populations were 0.645 0 , 0.537 5 , 0.518 3; the average of effec-tive number of alleles ( Ne) was 4.297 7 , 3.618 8 , 2.779 3; the average of observed heterozygosity ( He) was 0.633 4 , 0.592 0, 0.629 0; (3) Genetic differentiation between the parents was significant .Hybridization between O.potamophila and O.yaluensis was feasible .The inherited character between parents and hybrid follows the Mendelian inheritance rule and they were indeed hybrids in genetic material.(4)The hybrid progeny has a genetic distance of 1.0036 and 0.8361 from O.potamophila and O.yaluensis separately.The former was farther than the later , which showed that ngbrid progeng has an unequal heredity difference from its parents and a genetic inclination to its paternity.This was also supported by UPGMA.
