淡水渔业
淡水漁業
담수어업
FRESHWATER FISHERIES
2014年
5期
3-8
,共6页
于亚男%宋超%侯俊利%王妤%庄平
于亞男%宋超%侯俊利%王妤%莊平
우아남%송초%후준리%왕여%장평
虾虎鱼科%线粒体细胞C亚基(COI)%分子系统分类
蝦虎魚科%線粒體細胞C亞基(COI)%分子繫統分類
하호어과%선립체세포C아기(COI)%분자계통분류
Gobiidae%cytochrome oxidase subunit I ( COI) gene%molecular system classification
对采自长江口的7种虾虎鱼(Gobiidae)19个个体的线粒体COI基因进行测序,其他6种虾虎鱼的相应序列从Genbank中获得。序列分析结果显示7种虾虎鱼的COI基因部分序列密码子的第一位碱基的GC碱基含量最恒定,第三位碱基的GC含量最高,平均A+T的含量要高于G+C的含量,与多种鱼类的COI基因的碱基特点相似。运用MEGA5.0计算13种虾虎鱼的种间遗传距离,表明拉氏狼牙虾虎鱼(Oxyurichthy tentacularis)与孔虾虎鱼( Chaeturichthys stigmatias)归属于近盲虾虎鱼亚科,根据种间遗传距离与系统发育树的结构特征推测,触角沟虾虎鱼和矛尾虾虎鱼可能具有共同的起源。
對採自長江口的7種蝦虎魚(Gobiidae)19箇箇體的線粒體COI基因進行測序,其他6種蝦虎魚的相應序列從Genbank中穫得。序列分析結果顯示7種蝦虎魚的COI基因部分序列密碼子的第一位堿基的GC堿基含量最恆定,第三位堿基的GC含量最高,平均A+T的含量要高于G+C的含量,與多種魚類的COI基因的堿基特點相似。運用MEGA5.0計算13種蝦虎魚的種間遺傳距離,錶明拉氏狼牙蝦虎魚(Oxyurichthy tentacularis)與孔蝦虎魚( Chaeturichthys stigmatias)歸屬于近盲蝦虎魚亞科,根據種間遺傳距離與繫統髮育樹的結構特徵推測,觸角溝蝦虎魚和矛尾蝦虎魚可能具有共同的起源。
대채자장강구적7충하호어(Gobiidae)19개개체적선립체COI기인진행측서,기타6충하호어적상응서렬종Genbank중획득。서렬분석결과현시7충하호어적COI기인부분서렬밀마자적제일위감기적GC감기함량최항정,제삼위감기적GC함량최고,평균A+T적함량요고우G+C적함량,여다충어류적COI기인적감기특점상사。운용MEGA5.0계산13충하호어적충간유전거리,표명랍씨랑아하호어(Oxyurichthy tentacularis)여공하호어( Chaeturichthys stigmatias)귀속우근맹하호어아과,근거충간유전거리여계통발육수적결구특정추측,촉각구하호어화모미하호어가능구유공동적기원。
A total of nineteen specimens of seven species of Gobiidae derived from the Yangtze River were couected to se -quence mitochondrial COI genes.Sequences from homologous regions of six other species of this family were gathered from Genbank.The results showed that the most constant GC content was in the first base of codon and the highest GC content existed in the third base of codon .The average content of A +T was higher than the average content of G +C.The feature was similar to most fishes .Comparing the COI gene of thirteen species of Gobiidae with MEGA 5.0 , the taxonomic status of Odontamblyopus lacepedii and Trypauchen vagina were proved legitimately .The genetic distance between species and the structure feature of Phylogeny tree indicated the same origin of Oxyurichthy tentacularis and Chaeturichthys stigmatias.