CAJ | 학술논문

目的：研究白藜芦醇（Res）对动脉粥样硬化（AS）内皮细胞损伤的保护作用及机制。方法：应用溶血性磷脂酰胆碱（LPC）诱导的内皮细胞损伤模型，用双染流式细胞技术测定细胞凋亡率，TUNEL染色实验测定细胞凋亡数，MTT比色实验测定细胞活力，自动生化仪测定细胞培养基乳酸脱氢酶（LDH）水平，ELISA法测定TNF-琢含量，RT-PCR测定CGRP mRNA水平。结果：20滋mol·L-1浓度Res可有效地减弱由LPC诱导的细胞活力下降、培养基LDH水平升高及TNF-琢含量升高（P<0.01），Res可促进CGRP mRNA表达。结论：Res具有抗AS内皮损伤作用，其机制可能与通过激活VR1促进CGRP的合成和释放有关。
목적：연구백려호순（Res）대동맥죽양경화（AS）내피세포손상적보호작용급궤제。방법：응용용혈성린지선담감（LPC）유도적내피세포손상모형，용쌍염류식세포기술측정세포조망솔，TUNEL염색실험측정세포조망수，MTT비색실험측정세포활력，자동생화의측정세포배양기유산탈경매（LDH）수평，ELISA법측정TNF-탁함량，RT-PCR측정CGRP mRNA수평。결과：20자mol·L-1농도Res가유효지감약유LPC유도적세포활력하강、배양기LDH수평승고급TNF-탁함량승고（P<0.01），Res가촉진CGRP mRNA표체。결론：Res구유항AS내피손상작용，기궤제가능여통과격활VR1촉진CGRP적합성화석방유관。
This article was aimed to study the protective effects and mechanism of resveratrol (Res) on endothelial injury induced by atherosclerosis ( AS ) . Lysophosphatidylcholine ( LPC ) was applied to induce the model of injured endothelial cells. Flow cytometer was used to detect the rate of cell apoptosis. TUNEL staining was used to detect the cell apoptosis. MTT colorimetric test was used to detect the cell viability. Automatic biochemistry analyzer was used to measure the LDH level . ELISA was used to detect TNF-α level . RT-PCR was used to determine the mRNA levels of calcitonin gene-related peptide (CGRP). The results showed that Res with the concentration of 20 μmol/L can effectively slow down the degree of endothelial cell injury induced by LPC which caused the cell disability, high LDH level and high TNF-α level (P < 0.01). Res showed effects on mRNA expression of CGRP. It was concluded that Res can protect against endothelial injury induced by AS, the mechanism of which may be associated with accelerating CGRP synthesis and release by activating VR1.