CAJ | 학술논문

目的：研究渴络欣胶囊吸收入血的主要物质基础及特征。方法：采用HPLC-DAD体系，建立渴络欣胶囊大鼠血清的HPLC指纹图谱；差示分析渴络欣胶囊、空白血清及含药血清指纹图谱，获得大鼠口服渴络欣胶囊后血液中移行成分概况。结果：所建大鼠血清指纹图谱方法具有良好的精密度、重复性和稳定性；基于该方法分析渴络欣胶囊大鼠含药血清，共检出移行成分13个，其中原型成分9个、代谢成分4个，原型成分中包括芦荟大黄素及大黄酸。结论：吸收入血的13种成分可能是渴络欣胶囊发挥药效的主要物质基础。
목적：연구갈락흔효낭흡수입혈적주요물질기출급특정。방법：채용HPLC-DAD체계，건립갈락흔효낭대서혈청적HPLC지문도보；차시분석갈락흔효낭、공백혈청급함약혈청지문도보，획득대서구복갈락흔효낭후혈액중이행성분개황。결과：소건대서혈청지문도보방법구유량호적정밀도、중복성화은정성；기우해방법분석갈락흔효낭대서함약혈청，공검출이행성분13개，기중원형성분9개、대사성분4개，원형성분중포괄호회대황소급대황산。결론：흡수입혈적13충성분가능시갈락흔효낭발휘약효적주요물질기출。
This article was aimed to study the main effective substances and characteristics of Ke-Luo-Xin (KLX) capsule. HPLC-DAD system was applied in the establishment of HPLC finger prints of serum of rats after taking KLX capsule. And then, serum samples taken KLX capsule, blank blood serum, and single crude drugs were com-pared. Constituents absorbed into the serum were determined by HPLC-DAD system. The results showed that meth-ods for serum HPLC fingerprinting had good precision, reproducibility and stability. A total of 13 constituents migrat-ing to the blood were detected, of which 9 were prototype constituents, 4 were metabolites. Prototype constituents in-cluded aloeemodin and chrysophanic acid. It was concluded that 13 constituents migrating to the blood may be the main effective substances of KLX capsule.