CAJ | 학술논문

目的：采用ITS2序列对中药材半夏及其混伪品进行DNA条形码鉴定研究，为规范中药材半夏的市场流通，保障临床用药安全及疗效提供参考依据。方法：对半夏及其混伪品共59份样品，通过DNA提取及聚合酶链式反应（PCR）扩增其ITS2序列，并采用Mega6.0软件进行多序列比对，计算种内及种间K2P遗传距离，采用邻接（NJ）法构建NJ系统聚类树。采用相似性搜索法、最小距离法、NJ树法考察ITS2序列的鉴定能力。结果：半夏药材的ITS2序列长度为251 bp，应用相似性搜索法表明ITS2序列能够准确鉴定半夏药材及其混伪品；半夏种内最大K2P距离小于半夏与混伪品间的最小K2P距离；NJ树显示半夏药材可与其混伪品明显分开。结论：ITS2序列能准确、稳定鉴定中药材半夏药材及其混伪品。
목적：채용ITS2서렬대중약재반하급기혼위품진행DNA조형마감정연구，위규범중약재반하적시장류통，보장림상용약안전급료효제공삼고의거。방법：대반하급기혼위품공59빈양품，통과DNA제취급취합매련식반응（PCR）확증기ITS2서렬，병채용Mega6.0연건진행다서렬비대，계산충내급충간K2P유전거리，채용린접（NJ）법구건NJ계통취류수。채용상사성수색법、최소거리법、NJ수법고찰ITS2서렬적감정능력。결과：반하약재적ITS2서렬장도위251 bp，응용상사성수색법표명ITS2서렬능구준학감정반하약재급기혼위품；반하충내최대K2P거리소우반하여혼위품간적최소K2P거리；NJ수현시반하약재가여기혼위품명현분개。결론：ITS2서렬능준학、은정감정중약재반하약재급기혼위품。
In this study, the ITS2 sequence was used to identify Pinelliae Rhizoma and its adulterants to ensure its market circulation, clinical effect and safety. All genomic DNA from 59 samples were extracted successfully. The Kimura 2-Parameter (K2P) distances and NJ tree were calculated using software MEGA 6.0. The length of the ITS2 sequence of Pinelliae Rhizoma was 251 bp. The intraspecific genetic distance was smaller than the interspecific ones;The NJ tree indicated that Pinelliae Rhizoma distinguished from its adulterants obviously. The results showed that the ITS2 sequence can be used to distinguish Pinelliae Rhizoma from its adulterants accurately and stably.