CAJ | 학술논문

目的：对中药材远志及其混伪品进行分子鉴定，保障药材质量及用药安全。方法：对46份样品进行DNA提取，通过聚合酶链式反应（PCR）扩增其psbA-trnH序列并双向测序，应用CodonCode Aligner V 3.7.1对测序峰图校对拼接，去除低质量区和引物区，得到psbA-trnH序列，用MEGA 6.0对序列进行比对分析，基于K2P遗传距离构建系统聚类树。结果：通过相似性搜索法能准确鉴别远志及其混伪品，远志两个基原的种内最大K2P距离分别为0.004和0，均小于其与混伪品的种间最小K2P遗传距离（0.010和0.005），基于psbA-trnH序列的系统发育树可将远志药材及其混伪品明显区分开。结论：psbA-trnH序列能有效鉴别远志药材及其混伪品，为中药材的质量控制提供新方法。
목적：대중약재원지급기혼위품진행분자감정，보장약재질량급용약안전。방법：대46빈양품진행DNA제취，통과취합매련식반응（PCR）확증기psbA-trnH서렬병쌍향측서，응용CodonCode Aligner V 3.7.1대측서봉도교대병접，거제저질량구화인물구，득도psbA-trnH서렬，용MEGA 6.0대서렬진행비대분석，기우K2P유전거리구건계통취류수。결과：통과상사성수색법능준학감별원지급기혼위품，원지량개기원적충내최대K2P거리분별위0.004화0，균소우기여혼위품적충간최소K2P유전거리（0.010화0.005），기우psbA-trnH서렬적계통발육수가장원지약재급기혼위품명현구분개。결론：psbA-trnH서렬능유효감별원지약재급기혼위품，위중약재적질량공제제공신방법。
In this study,Polygalae radix and its adulterants were identified by psbA-trnH sequence.The genomic DNA was extracted from forty-six samples, the psbA-trnH sequences were amplified and sequenced Bi-directionally, and then assembled sequences by Codoncode Aligner V 3.7.1. The genetic distances were computed by kimura 2-parameter (K2P) model, and the Neighbor-Joining tree was constructed by MEGA 6.0. Results showed that minimum intra-specific K2P distance of Polygala tenuifolia and Polygala sibirica were 0.004 and 0, which were smaller than the maximum intra-specific K2P. The NJ tree showed Polygalae radix can be distinguished from its adulterants by psbA-trnH sequences. Therefore, using psbA-trnH sequences can distinguish Polygalae radix from its adulterants.