CAJ | 학술논문

目的：研究鼻黏膜嗅鞘细胞在不同条件尤其在正常脑脊液中的生长情况，以利于实施自体嗅鞘细胞移植。方法设置5种不同培养条件进行比较，了解脑脊液培养条件下细胞的贴壁、增殖、形态学及细胞纯度的变化。结果普通培养基培养一周后改用脑脊液培养对细胞的增殖能力不造成很大影响，细胞形态及纯度也无明显变化。结论使用脑脊液培养作为自体细胞移植前的过渡将规避伦理学风险，临床是可行的。
목적：연구비점막후초세포재불동조건우기재정상뇌척액중적생장정황，이리우실시자체후초세포이식。방법설치5충불동배양조건진행비교，료해뇌척액배양조건하세포적첩벽、증식、형태학급세포순도적변화。결과보통배양기배양일주후개용뇌척액배양대세포적증식능력불조성흔대영향，세포형태급순도야무명현변화。결론사용뇌척액배양작위자체세포이식전적과도장규피윤리학풍험，림상시가행적。
ObjectiveStudy of growth of nasal mucosa OECS in the normal CSF will be useful for carrying out clinic study of OECS autotransplantation.Methods 5 different culture conditions were compared to find out the change of OECS adherence, proliferation,morphology and purity under the CSF culture condition.ResultsThe culture turning common medium into CSF after 1 CSF after 1 week didn’t cause significant influence of cells proliferation ability.Their morphology and purity didn’t experience much change, either.ConclusionAs a method of passing over,using CSF culture before OECS autotransplantation will avoid ethical risk, which will be feasible in clinic.