CAJ | 학술논문

G蛋白偶联受体( GPCRs)是生物体内重要膜受体，通过激活三聚体 G 蛋白参与各种细胞内信号转导，在药物研发中具有重要的作用。从舞毒蛾无参照全转录本文库中鉴定获得 GPCR 家族中眼白化 I 型全长基因(命名为 LdOA1)，该基因全长453 bp，编码150个氨基酸，分子质量为17.54 kDa，理论等电点(pI)为9.76。进化树分析表明 LdOA1蛋白与帝王蝶的亲缘关系较近。实时荧光定量 PCR 分析表明舞毒蛾3龄幼虫在溴氰菊酯、甲萘威和氧化乐果胁迫下 LdOA1的表达均表现为显著抑制。
G단백우련수체( GPCRs)시생물체내중요막수체，통과격활삼취체 G 단백삼여각충세포내신호전도，재약물연발중구유중요적작용。종무독아무삼조전전록본문고중감정획득 GPCR 가족중안백화 I 형전장기인(명명위 LdOA1)，해기인전장453 bp，편마150개안기산，분자질량위17.54 kDa，이론등전점(pI)위9.76。진화수분석표명 LdOA1단백여제왕접적친연관계교근。실시형광정량 PCR 분석표명무독아3령유충재추청국지、갑내위화양화악과협박하 LdOA1적표체균표현위현저억제。
The G protein coupled receptors ( GPCRs) ,known as transmembrane receptors,play an important role in drug research and development by activating G protein involved in inside signal transduction pathways. In present study, the full length cDNA of GPCR (namely LdOA1) was isolated from Lymantria dispar transcriptome. The open reading frame ( ORF) of LdOA1 was 453 bp encoding a protein of 150 amino acid residues with the molecular mass of 17. 54 kDa and theoretical pI of 9. 76. Phylogenetic analysis of GPCR proteins showed LdOA1 of L. dispar clustered into a group with Danaus plexippus. We further investigated the expression of LdOA1 in 3rd instar L. dispar larvae under deltamethrin, carbaryl and omethoate stresses using real-time PCR. The results showed that LdOA1 in L. dispar was obviously inhibited by deltamethrin,carbaryl and omethoate.