林业科学
林業科學
임업과학
SCIENTIA SILVAE SINICAE
2014年
8期
76-81
,共6页
刘政捷%林源%周明兵%汤定钦
劉政捷%林源%週明兵%湯定欽
류정첩%림원%주명병%탕정흠
毛竹%微卫星%遗传多样性%交配系统%异交率
毛竹%微衛星%遺傳多樣性%交配繫統%異交率
모죽%미위성%유전다양성%교배계통%이교솔
Phyllostachys edulis%microsatellite%genetic diversity%mating system%rate of outcrossing
选用自行开发引物的、具有较高遗传多态性的8个微卫星位点,分析来自独立的3株开花毛竹半同胞家系各50个子代个体。结果表明:位于3个不同地点的开花植株都能够通过与外源花粉杂合产生具有不同的微卫星基因型特点的半同胞家系子代,各个家系子代内均存有一定的遗传多样性,估算的平均异交率约为0.089。这说明毛竹主要以自交为主,并兼顾有一定比例的异交以确保其后代种群的遗传多样性。
選用自行開髮引物的、具有較高遺傳多態性的8箇微衛星位點,分析來自獨立的3株開花毛竹半同胞傢繫各50箇子代箇體。結果錶明:位于3箇不同地點的開花植株都能夠通過與外源花粉雜閤產生具有不同的微衛星基因型特點的半同胞傢繫子代,各箇傢繫子代內均存有一定的遺傳多樣性,估算的平均異交率約為0.089。這說明毛竹主要以自交為主,併兼顧有一定比例的異交以確保其後代種群的遺傳多樣性。
선용자행개발인물적、구유교고유전다태성적8개미위성위점,분석래자독립적3주개화모죽반동포가계각50개자대개체。결과표명:위우3개불동지점적개화식주도능구통과여외원화분잡합산생구유불동적미위성기인형특점적반동포가계자대,각개가계자대내균존유일정적유전다양성,고산적평균이교솔약위0.089。저설명모죽주요이자교위주,병겸고유일정비례적이교이학보기후대충군적유전다양성。
Mating system is the basic property of plant populations and biological foundation for germplasm preservation as well as genetic improvement. In this study we applied developed 8 microsatellite loci with high polymorphism to analyze 50 half-sib progenies from each of 3 independent flowering culms of Phyllostachys edulis. The results showed that flowering culms in three different locations were fertilized with foreign pollen,which generated progenies with different microsatellite genotypes and genetic diversity. The overall estimates of outcrossing rate tm of single-locus and ts of multi-locus for the three culms were 0. 089,indicating that the reproductive system of P. edulis predominantly involved in self-fertilization with a certain proportion of outcrossing to produce genetic diverse progenies. This is the first report so far on estimation of outcrossing rate for monopodial bamboo species such as P. edulis by DNA molecular markers.