CAJ | 학술논문

利用光学和电子显微镜技术，对巴西橡胶树树皮中单宁细胞的形态结构和发育过程进行研究。结果表明：除伴胞外，次生韧皮部中几乎所有的薄壁细胞都含有单宁类物质，乳管周围的薄壁细胞中尤其易积累单宁，石细胞和周皮中的木栓层以及栓内层细胞中也有大量单宁积累。电镜下，单宁细胞中央大液泡中积累的单宁可分为絮状、不规则状、颗粒状和板块状4种类型。在单宁细胞发育过程中，单宁首先在细胞质中产生，中央大液泡形成后则成为积累单宁的主要场所并最终被单宁类物质充满。机械伤害、强割、外施乙烯利以及茉莉酸( JA )都能大幅提升单宁类物质的积累，可见橡胶树对这些因子都能做出防御性应答。
이용광학화전자현미경기술，대파서상효수수피중단저세포적형태결구화발육과정진행연구。결과표명：제반포외，차생인피부중궤호소유적박벽세포도함유단저류물질，유관주위적박벽세포중우기역적루단저，석세포화주피중적목전층이급전내층세포중야유대량단저적루。전경하，단저세포중앙대액포중적루적단저가분위서상、불규칙상、과립상화판괴상4충류형。재단저세포발육과정중，단저수선재세포질중산생，중앙대액포형성후칙성위적루단저적주요장소병최종피단저류물질충만。궤계상해、강할、외시을희리이급말리산( JA )도능대폭제승단저류물질적적루，가견상효수대저사인자도능주출방어성응답。
The morphological structure and development of tannin cells in bark of Hevea brasiliensis were studied by using transmission electron microscopy and light microscopy. The results showed that tannin-like materials distributed in all kinds of parenchyma cells in secondary phloem except for companion cells. The tannin-like materials were present in large amounts in the parenchyma cells around the laticifers,the stone cells,cork layer cells and phelloderm cells. Under the electron microscope,four forms of tannin-like materials,i. e. ,flocculent,irregular,granular and plate-like,could be distinguished in the large central vacuoles of tannin cells. During the course of development of tannin cell,tannin-like materials firstly deposited into small vesicles in cytoplasm,then stored mainly in the large central vacuole along with the synthesis of tannins. As a result,the tannin-like materials filled the large central vacuole of the mature tannin cells. The accumulation of tannin-like materials was largely enhanced by mechanic wounding,over-exploitation,and application of either Ethrel or Jasmonate ( JA) ,reflecting the response of rubber tree to various stress factors.