CAJ | 학술논문

为考查洗涤厂热废水排放口土壤在常年人为热环境下的可培养嗜热菌的多样性，采集洗涤厂热废水排放口附近的湿热泥土，应用不同培养基分离纯化泥土中的可培养嗜热菌，并对获得的纯培养细菌进行16S rDNA鉴定和系统发育相关分析。结果表明：分离纯化得到的29株不同表型的纯培养菌株均属于坚壁菌门芽孢杆菌纲，归属于5个属11个种，分别是地芽孢杆菌属 Geobacillus 3个种，芽孢杆菌属 Bacillus 3个种，无氧芽孢杆菌属 Anoxybacillus 3个种，解脲芽孢杆菌属Ureibacillus 1个种和高温放线菌属 Thermoactinomyces 1个种，属种多样性丰富，菌株资源具有潜在开发应用价值。
위고사세조엄열폐수배방구토양재상년인위열배경하적가배양기열균적다양성，채집세조엄열폐수배방구부근적습열니토，응용불동배양기분리순화니토중적가배양기열균，병대획득적순배양세균진행16S rDNA감정화계통발육상관분석。결과표명：분리순화득도적29주불동표형적순배양균주균속우견벽균문아포간균강，귀속우5개속11개충，분별시지아포간균속 Geobacillus 3개충，아포간균속 Bacillus 3개충，무양아포간균속 Anoxybacillus 3개충，해뇨아포간균속Ureibacillus 1개충화고온방선균속 Thermoactinomyces 1개충，속충다양성봉부，균주자원구유잠재개발응용개치。
To investigate the diversity of mesophilic bacteria resulting from constant thermal conditions , the soil sample was collected at outfall of thermal wastewater from laundry factory .The culturable mesophilic bacteria were isolated with different medium to obtain pure culture , identified by 16S rDNA and the data were analyzed in phylogenetic tree .The results showed that the 29 strains in pure culture were obtained from the thermal wastewater outfall soil with different phenotype .The strains were classified into Bacilli ,attributed to 5 genus and 11 species , including three species of Geobacillus ,three species of Bacillus ,three species of Anoxybacillus ,one species of Ureibacillus ,and one species of Thermoactinomyces .The diversity of species was rich ,and the resources of species would have potential in practical application .