CAJ | 학술논문

活性氧ROS是生理过程必要的代谢产物，适量的ROS起到信号转导作用，一旦过量会对细胞、蛋白质和DN A 造成损伤．在顺序注射-阀上实验室-化学发光系统内，优化并评价了鲁米诺-Co2＋-ROS和鲁米诺-HRP-ROS 2种化学发光体系对ROS的检测条件和分析性能，并将其应用于肝细胞和植物叶片ROS的测定．结果表明，顺序注射-阀上实验室-ROS-化学发光检测法具有自动化程度高、分析速度快、重现性好和试剂试样用量少的显著特点，其中鲁米诺-Co2＋-ROS 体系灵敏度高，相对发光强度与ROS浓度在0．5～1000 nmol · L -1范围内的回归方程为Y ＝49X＋1700（R2＝0．9924），检出限为0．27 nmol · L -1，对细胞和植物组织中 ROS 的回收率在95％～104％之间，结果令人满意．
활성양ROS시생리과정필요적대사산물，괄량적ROS기도신호전도작용，일단과량회대세포、단백질화DN A 조성손상．재순서주사-벌상실험실-화학발광계통내，우화병평개료로미낙-Co2＋-ROS화로미낙-HRP-ROS 2충화학발광체계대ROS적검측조건화분석성능，병장기응용우간세포화식물협편ROS적측정．결과표명，순서주사-벌상실험실-ROS-화학발광검측법구유자동화정도고、분석속도쾌、중현성호화시제시양용량소적현저특점，기중로미낙-Co2＋-ROS 체계령민도고，상대발광강도여ROS농도재0．5～1000 nmol · L -1범위내적회귀방정위Y ＝49X＋1700（R2＝0．9924），검출한위0．27 nmol · L -1，대세포화식물조직중 ROS 적회수솔재95％～104％지간，결과령인만의．
Reactive oxygen species (ROS) are physiological metabolites ,appropriate amount ROS play an essential role in signal transduction ,but excess ROS can damage cellular components such as pro-teins ,lipids ,and DNA .In this work ,employing sequential injection lab-on-valve chemiluminescent technique(SIA-LOV-CL) ,we explored and optimized the factors influencing Luminol-Co2+-ROS and Luminol-HRP-ROS chemiluminescent system ,respectively .We also applied the proposed method for the determination of ROS in Chang liver cells and plant tissue .The results showed that SIA-LOV-CL system for ROS determination not only possessed high sensitivity and stability ,but also minimized sample and reagent consumption .The relative CL intensity for Luminol-Co2+-ROS system was linear with ROS concentration in the range of 0 .5~1000 nmol · L -1 ,and a 0 .27 nmol · L -1 detection limit was achieved .The recoveries for the determination of ROS in Chang liver cells and plant tissue were in the range of 95% ~104% .