CAJ | 학술논문

在金表面上制备 L-半胱氨酸（L-Cys）自组装单分子膜（SAMs）已引起人们的广泛兴趣．但是，我们发现，不同自组装溶液对SAMs的形成具有很大影响．利用偏振模式傅立叶变换红外反射吸收光谱对分别在磷酸盐缓冲溶液（PBS ）、醋酸盐缓冲溶液（ABS ）以及盐酸与氢氧化钠混合溶液中制备的 L-Cys SAMs的结构进行了研究．结果表明，在不同溶液中制备 L-Cys SAMs的最佳酸度条件不同，在ABS中为pH 5．0，在盐酸与氢氧化钠混合溶液中为pH 6．0，而在PBS中 L-Cys SAM s的表面密度随着pH的增加而增大．就所研究的3种溶液而言，在优化条件下，在ABS中制备的 L-Cys SAM s的表面密度最大，因而其表面结构最完美．本研究为进一步制备免疫传感器、酶传感器等生物传感器提供了有利的技术支持．
재금표면상제비 L-반광안산（L-Cys）자조장단분자막（SAMs）이인기인문적엄범흥취．단시，아문발현，불동자조장용액대SAMs적형성구유흔대영향．이용편진모식부립협변환홍외반사흡수광보대분별재린산염완충용액（PBS ）、작산염완충용액（ABS ）이급염산여경양화납혼합용액중제비적 L-Cys SAMs적결구진행료연구．결과표명，재불동용액중제비 L-Cys SAMs적최가산도조건불동，재ABS중위pH 5．0，재염산여경양화납혼합용액중위pH 6．0，이재PBS중 L-Cys SAM s적표면밀도수착pH적증가이증대．취소연구적3충용액이언，재우화조건하，재ABS중제비적 L-Cys SAM s적표면밀도최대，인이기표면결구최완미．본연구위진일보제비면역전감기、매전감기등생물전감기제공료유리적기술지지．
L-Cysteine (Cys) is one of most the interesting thiolated amino acid candidates for self-as-sembled monofilm (SAMs) formation on the gold electrode .However ,we have found that the forma-tion of Cys SAMs would be effected by the type of aqueous media .In this paper ,the structures of Cys SAM s prepared on gold substrate in phosphate buffered solution (PBS ) ,acetate buffer solution (ABS) ,solution of sodium chloride and sodium hydroxide (NaOH/HCl) have been studied using Po-larization-modulation Fourier transform infrared reflection-absorption spectroscopy (PM-FT-IRRAS) respectively .The results showed that the optimum cyclic condition for preparation of Cys SAMs on the gold surface prepared in ABS was pH5 .0 ,in NaOH/HCl was pH6 .0 ,and in PBS the Cys SAM s density increases with the increase of pH .The Cys SAMs prepared in ABS has a maximum surface density in the three solutions ,so the structure of the Cys SAMs is most perfect .Therefore ,ABS is an ideal self assembling solution .T he results provide technical support for the preparation of chemical sensors ,immunosensors and enzyme sensors .