CAJ | 학술논문

目的：了解毒力基因在泛耐药铜绿假单胞菌中的分布情况，并分析其同源性。方法采用 PCR 方法检测毒力基因exoU 和 exoS ，用 BOX-PCR 指纹图谱分析技术进行同源性分析。结果53株临床分离的泛耐药铜绿假单胞菌，其中40株毒力基因为 exoS＋/exoU-型，10株为 exoU＋/exoS -基因型，1株为 exoS ＋/exoU＋基因型，2株为 exoS -/exo U -基因型。BOX-PCR 结果显示，41株 exoS＋菌株分为24种基因型，11株 exoU＋菌株分为7种基因型。结论临床分离的泛耐药铜绿假单胞菌，毒力基因的携带率高，BOX-PCR 指纹图谱分析技术适合铜绿假单胞菌的同源性分析，可为医院感染的控制与管理提供病原学基因分型资料。
목적：료해독력기인재범내약동록가단포균중적분포정황，병분석기동원성。방법채용 PCR 방법검측독력기인exoU 화 exoS ，용 BOX-PCR 지문도보분석기술진행동원성분석。결과53주림상분리적범내약동록가단포균，기중40주독력기인위 exoS＋/exoU-형，10주위 exoU＋/exoS -기인형，1주위 exoS ＋/exoU＋기인형，2주위 exoS -/exo U -기인형。BOX-PCR 결과현시，41주 exoS＋균주분위24충기인형，11주 exoU＋균주분위7충기인형。결론림상분리적범내약동록가단포균，독력기인적휴대솔고，BOX-PCR 지문도보분석기술괄합동록가단포균적동원성분석，가위의원감염적공제여관리제공병원학기인분형자료。
Objective To study the prevalence and sequence homology of virulence genes exoU and exoS in 53 strains of pan-drug-resistant Pseudomonas aeruginosa .Methods The virulence genes exoU and exoS were detected by PCR.Sequence homo-logy was analyzed by BOX-PCR.Results Of the 53 clinical isolates of pandrug-resistant Pseudomonas aeruginosa ,the exoS+/exoU- genotype was identified in 40 strains,exoU+/exoS - genotype in 10 strains,exoS +/exoU+ genotype in 1 strain, and exoS-/exoU- genotype in 2 strains.BOX-PCR results showed that 41 exoS+ isolates belonged to 24 genotypes,and 11 exoU+ strains could be grouped into 7 genotypes.Conclusions The prevalence of virulence genes is high in clinical isolates of pandrug-resistant Pseudomonas aeruginosa .BOX-PCR fingerprint analysis combined with sequence homology analysis is help-ful for effective monitoring and control of hospital pandrug-resistant pseudomonas aeruginosa infection.