CAJ | 학술논문

从疑似牛传染性鼻气管炎病毒感染的牛鼻腔中分离出1株病毒，通过PCR方法和MDBK细胞病变观察对其进行鉴定，确定该分离株为牛传染性鼻气管炎毒株。通过对该毒株的增殖条件和甲醛灭活条件的优化研究，为制备牛传染性鼻气管炎细胞源灭活疫苗提供参考依据。结果表明该毒株接毒18 h后病变细胞达70%开始收毒，病毒液在0.2%的甲醛37℃灭活36 h灭活效果最好。
종의사우전염성비기관염병독감염적우비강중분리출1주병독，통과PCR방법화MDBK세포병변관찰대기진행감정，학정해분리주위우전염성비기관염독주。통과대해독주적증식조건화갑철멸활조건적우화연구，위제비우전염성비기관염세포원멸활역묘제공삼고의거。결과표명해독주접독18 h후병변세포체70%개시수독，병독액재0.2%적갑철37℃멸활36 h멸활효과최호。
A virus isolated from bovine nasal swab suspected infectious bovine rhinotracheitis virus infection by PCR and the MDBK cell lesions observed to identify it. The isolates strains of infectious bovine rhinotracheitis. Optimization of the strain proliferation conditions and formaldehyde inactivated conditions,to provide a reference for the preparation of infectious bovine rhinotracheitis cell-derived inactivated vaccine. The results show that the strain inoculation 18 h diseased cells to fight 70% began to collect the drugs,the virus was inactivated in 0.2%formaldehyde 37℃for 36 h inactivated the best.