CAJ | 학술논문

从美国华盛顿州圣璜岛海域的8种海绵样品的120株共附生微生物中,筛选出11株细菌的粗提物能有效抑制硅藻附着。其中683号菌株活性最高,对5种受试硅藻的平均抑制率达到98.0%。结合形态学特征和扫描电镜观察,以及16S rDNA序列分析,鉴定该菌株为短小芽孢杆菌Bacillus pumilus。并采用正交试验对该菌株进行发酵条件优化,结合粗提物的产量和抗硅藻附着活性进行统计学分析,确定了该菌株的最佳发酵条件是：发酵温度25℃, pH 7.5, NaCl浓度19.45 g/L,蛋白胨浓度5 g/L。
종미국화성돈주골황도해역적8충해면양품적120주공부생미생물중,사선출11주세균적조제물능유효억제규조부착。기중683호균주활성최고,대5충수시규조적평균억제솔체도98.0%。결합형태학특정화소묘전경관찰,이급16S rDNA서렬분석,감정해균주위단소아포간균Bacillus pumilus。병채용정교시험대해균주진행발효조건우화,결합조제물적산량화항규조부착활성진행통계학분석,학정료해균주적최가발효조건시：발효온도25℃, pH 7.5, NaCl농도19.45 g/L,단백동농도5 g/L。
11 strains were screened from 120 epibiotic strains isolated from 8 sponge species from San Juan Island, USA. These 11 strains were able to efficiently inhibit diatom adhesion. One strain named 683 had the highest acti-vity in inhibiting diatom adhesion with an average inhibition of 98.0%on the 5 tested diatoms. The strain 683 was identified to be Bacillus pumilus based on analysis of colony morphologyand scanning electron microscope (SEM), as well as 16S rDNA sequence analysis. The optimum fermentation conditions of strain 683 were further optimized through orthogonal test. Through the statistical analysis of crude extract production and diatom adhesion inhibitory activity, the optional fermentation conditions for strain 683 were:fermentation temperature of 25℃, pH 7.5, NaCl concentration of 19.45 g/L, and peptone concentration of 5 g/L.