CAJ | 학술논문

目的：观察电针次髎、中极、三阴交穴对骶髓损伤后神经源性膀胱(逼尿肌无反射型)大鼠尿流动力学以及膀胱组织中caspase-3表达的影响。方法雌性Sprague-Dawley大鼠40只，随机抽取10只为空白组，其余造模后随机分为模型组、穴位组和对照点组，每组10只。术后第15天起按组分别施以7次治疗。测量术后第14天及干预治疗7次后1 d的大鼠尿流动力学参数；治疗结束后取膀胱组织采用ELISA法检测在膀胱组织中caspase-3的表达。结果电针治疗后大鼠最大膀胱容量显著降低(P<0.001)，膀胱顺应性显著降低(P<0.001)，逼尿肌组织中caspase-3表达明显降低(P<0.01)，充盈性膀胱内压升高(P<0.05)，且穴位组疗效优于对照点组(P<0.05)。结论电针次髎、中极、三阴交穴可改善骶髓损伤后神经源性膀胱大鼠尿流动力学参数，降低膀胱组织中cas-pase-3的表达。
목적：관찰전침차료、중겁、삼음교혈대저수손상후신경원성방광(핍뇨기무반사형)대서뇨류동역학이급방광조직중caspase-3표체적영향。방법자성Sprague-Dawley대서40지，수궤추취10지위공백조，기여조모후수궤분위모형조、혈위조화대조점조，매조10지。술후제15천기안조분별시이7차치료。측량술후제14천급간예치료7차후1 d적대서뇨류동역학삼수；치료결속후취방광조직채용ELISA법검측재방광조직중caspase-3적표체。결과전침치료후대서최대방광용량현저강저(P<0.001)，방광순응성현저강저(P<0.001)，핍뇨기조직중caspase-3표체명현강저(P<0.01)，충영성방광내압승고(P<0.05)，차혈위조료효우우대조점조(P<0.05)。결론전침차료、중겁、삼음교혈가개선저수손상후신경원성방광대서뇨류동역학삼수，강저방광조직중cas-pase-3적표체。
Objective To explore the effects of electrical acupuncture at Ciliao (BL32), Zhongji (RN03), Sanyinjiao (SP06) acupoints on the expression of caspase-3 in bladder tissue and the urodynamics of rats with neurogenic bladder (detrusor areflexia type) after sacral spinal cord injury. Methods 10 rats were randomly selected as blank control group from 40 female Sprague-Dawley rats. The other 30 rats were randomly divided into model group, acupoint group and control group after modeling, with 10 rats in each group. The urodynamics was mea-sured on the 14th and 22nd day after modeling. The control group and acupoint group received electrical acupuncture 7 times at the acu-points and other points nearby, respectively, from the 15th day. The expression of caspase-3 in bladder tissue was detected with en-zyme-linked immuno sorbent assay (ELISA) method. Results Compared with the model group, the maximum bladder capacity, bladder com-pliance and expression of caspase-3 in bladder tissue significantly decreased after treatment (P<0.01), the filling bladder pressure significant-ly increased (P<0.05) in the acupoint group. And the acupuncture group was better than the control group (P<0.05). Conclusion Electrical acupuncture at Ciliao, Zhongji and Sanyinjiao can reduce the maximum bladder capacity in neurogenic bladder rats and inhabit the expres-sion of caspase-3 in bladder tissue.