南方医科大学学报
南方醫科大學學報
남방의과대학학보
JOURNAL OF SOUTHERN MEDICAL UNIVERSITY
2013年
12期
1827-1830
,共4页
徐燕萍%张清%湛学军%谢大泽%戴革%杨慧
徐燕萍%張清%湛學軍%謝大澤%戴革%楊慧
서연평%장청%담학군%사대택%대혁%양혜
鸡卵黄%特异性转移因子%乙型肝炎病毒%口服液
鷄卵黃%特異性轉移因子%乙型肝炎病毒%口服液
계란황%특이성전이인자%을형간염병독%구복액
egg yolk%transfer factor%hepatitis B virus%oral solution
目的:制备鸡卵黄乙肝病毒特异性转移因子(EYHBV-STF)口服液,并鉴定其活性,探索一种新型、有效治疗乙肝的免疫调节剂。方法采用乙肝疫苗免疫母鸡,从卵黄中提取乙型肝炎病毒特异转移因子(HBV-STF),进行理化特性等检测。采用白细胞粘附抑制试验检测转移因子的免疫活性,将其制成口服液,经小鼠灌胃后,迟发型皮肤超敏反应试验鉴定其体内特异性细胞免疫活性,免疫脏器指数鉴定其非特异性免疫活性。同时分别与经注射或口服途径的EYHBV-STF组、传统方法制备的猪脾乙肝特异性转移因子组、猪脾非特异性转移因子组及未经免疫的鸡卵黄提取液组进行比较。结果EYHBV-STF口服液各项理化特性及安全实验指标符合TF生物制品标准,体外实验能明显抑制正常小鼠白细胞黏附,体内实验能显著增强小鼠足垫肿胀度反应,提示该制品可将供体的乙肝抗原特异性皮肤迟发型超敏反应转移给受体,并能够显著提高免疫脏器指数(P<0.01),与注射EYHBV-STF、传统方法制备的注射、口服PHBV-STF对照组比较,各项实验指标结果相近(P>0.05)。结论口服EYHBV-STF与注射途径同样具有依赖乙肝抗原的特异性细胞免疫活性,可明显增强正常小鼠特异性细胞免疫功能。
目的:製備鷄卵黃乙肝病毒特異性轉移因子(EYHBV-STF)口服液,併鑒定其活性,探索一種新型、有效治療乙肝的免疫調節劑。方法採用乙肝疫苗免疫母鷄,從卵黃中提取乙型肝炎病毒特異轉移因子(HBV-STF),進行理化特性等檢測。採用白細胞粘附抑製試驗檢測轉移因子的免疫活性,將其製成口服液,經小鼠灌胃後,遲髮型皮膚超敏反應試驗鑒定其體內特異性細胞免疫活性,免疫髒器指數鑒定其非特異性免疫活性。同時分彆與經註射或口服途徑的EYHBV-STF組、傳統方法製備的豬脾乙肝特異性轉移因子組、豬脾非特異性轉移因子組及未經免疫的鷄卵黃提取液組進行比較。結果EYHBV-STF口服液各項理化特性及安全實驗指標符閤TF生物製品標準,體外實驗能明顯抑製正常小鼠白細胞黏附,體內實驗能顯著增彊小鼠足墊腫脹度反應,提示該製品可將供體的乙肝抗原特異性皮膚遲髮型超敏反應轉移給受體,併能夠顯著提高免疫髒器指數(P<0.01),與註射EYHBV-STF、傳統方法製備的註射、口服PHBV-STF對照組比較,各項實驗指標結果相近(P>0.05)。結論口服EYHBV-STF與註射途徑同樣具有依賴乙肝抗原的特異性細胞免疫活性,可明顯增彊正常小鼠特異性細胞免疫功能。
목적:제비계란황을간병독특이성전이인자(EYHBV-STF)구복액,병감정기활성,탐색일충신형、유효치료을간적면역조절제。방법채용을간역묘면역모계,종란황중제취을형간염병독특이전이인자(HBV-STF),진행이화특성등검측。채용백세포점부억제시험검측전이인자적면역활성,장기제성구복액,경소서관위후,지발형피부초민반응시험감정기체내특이성세포면역활성,면역장기지수감정기비특이성면역활성。동시분별여경주사혹구복도경적EYHBV-STF조、전통방법제비적저비을간특이성전이인자조、저비비특이성전이인자조급미경면역적계란황제취액조진행비교。결과EYHBV-STF구복액각항이화특성급안전실험지표부합TF생물제품표준,체외실험능명현억제정상소서백세포점부,체내실험능현저증강소서족점종창도반응,제시해제품가장공체적을간항원특이성피부지발형초민반응전이급수체,병능구현저제고면역장기지수(P<0.01),여주사EYHBV-STF、전통방법제비적주사、구복PHBV-STF대조조비교,각항실험지표결과상근(P>0.05)。결론구복EYHBV-STF여주사도경동양구유의뢰을간항원적특이성세포면역활성,가명현증강정상소서특이성세포면역공능。
Objective To prepare the oral solution of egg yolk hepatitis B virus (HBV)-specific transfer factor (EYHBV-TF) and evaluate its immunological activity as an immune regulator against hepatitis B. Methods From hens immunized with the Hepatitis B vaccine the egg yolk was isolated to extract the specific transfer factor EYHBV-TF, and its physicochemical properties were examined. Leukocyte adhesion inhibition test (LAI) was performed to detect the immunogenic activity of EYHBV-TF. The solution of EYHBV-TF was then administered orally in normal mice, and the specific cellular immune activity induced was assayed with delayed type skin hypersensitivity test (DTH), with the non-specific immune activity assessed with immune organ index. The immune responses induced by oral EYHBV-STF solution were compared with those by EYHBV-STF injection and by different dosages (injection and oral) of porcine spleen HBV-specific transfer factor (PSHBV-STF), porcine spleen nonspecific transfer factor, and egg yolk extracts from non-immunized hens. Results The prepared EYHBV-STF oral solution, which met the standards for biological products, could inhibit leukocyte adhesion in vitro and significantly enhance mouse foot pad swelling, demonstrating its capability of transferring antigen-specific delayed type hypersensitivity reactions to naive recipient. EYHBV-STF oral solution also significantly improved the immune organ index in mice (P<0 01) with similar effects to those caused by EYHBV-STF injections and by PSHBV-STF injection and oral solution. Conclusion Orally administered EYHBV-STF and EYHBV-STF injection both possess hepatitis B antigen-specific cellular immune activity and can significantly enhance specific cellular immune responses.