CAJ | 학술논문

目的：通过检测STAT3及P-STAT3在APPswe/PS△E9双转基因阿尔茨海默病（AD）小鼠脑组织中的表达，探讨其在AD发病过程中的可能作用。方法采用免疫组化法检测APPswe/PS△E9双转基因AD小鼠及对照小鼠脑组织中STAT3和P-STAT3的表达。结果STAT3和P-STAT3表达于脑组织的不同部位。STAT3在转基因AD小鼠和对照小鼠的大脑皮层、基底前脑、海马及小脑中的阳性表达率分别为93.75%、87.50%，87.50%、43.75%，81.25%、37.50%，62.50%、0.00%，其中差异有统计学意义的是基底前脑、海马及小脑中的表达（P<0.05）；P-STAT3在转基因AD小鼠和对照小鼠的大脑皮层、基底前脑、海马及小脑中的阳性表达率分别为0.00%、0.00%，68.75%、0.00%，62.50%、12.50%，43.75%、0.00%，其中差异有统计学意义的是基底前脑、海马及小脑中的表达（P<0.05）；且STAT3和P-STAT3呈正相关（P<0.05）。结论STAT3和P-STAT3在转基因AD小鼠的基底前脑、海马及小脑中存在高表达，其可能参与了AD发病的病理过程。
목적：통과검측STAT3급P-STAT3재APPswe/PS△E9쌍전기인아이자해묵병（AD）소서뇌조직중적표체，탐토기재AD발병과정중적가능작용。방법채용면역조화법검측APPswe/PS△E9쌍전기인AD소서급대조소서뇌조직중STAT3화P-STAT3적표체。결과STAT3화P-STAT3표체우뇌조직적불동부위。STAT3재전기인AD소서화대조소서적대뇌피층、기저전뇌、해마급소뇌중적양성표체솔분별위93.75%、87.50%，87.50%、43.75%，81.25%、37.50%，62.50%、0.00%，기중차이유통계학의의적시기저전뇌、해마급소뇌중적표체（P<0.05）；P-STAT3재전기인AD소서화대조소서적대뇌피층、기저전뇌、해마급소뇌중적양성표체솔분별위0.00%、0.00%，68.75%、0.00%，62.50%、12.50%，43.75%、0.00%，기중차이유통계학의의적시기저전뇌、해마급소뇌중적표체（P<0.05）；차STAT3화P-STAT3정정상관（P<0.05）。결론STAT3화P-STAT3재전기인AD소서적기저전뇌、해마급소뇌중존재고표체，기가능삼여료AD발병적병리과정。
Objective To detect the expression of signal transducer and activator of transcription 3 (STAT3) and P-STAT3 in the brain of the APPswe/PS△E9 double transgenic mouse model of Alzhaimer's disease (AD) and invesitgate their possible role in AD. Methods APPswe/PS △E9 double transgenic mice and control mice were examined for cerebral STAT3 and P-STAT3 expressions using immunothistochemistry. Results STAT3 and P-STAT3 were expressed in the different regions of mouse brain. In the transgenic mice and the control mice, the positivity rates of STAT3 were 93.75%and 87.50%in the cerebral cortex, 87.50% and 43.75% in the basal forebrain, 81.25% and 37.50% in the hippocampus, and 62.50% and 0.00% in the cerebellum, respectively, showing significant differences between the mice in the STAT3 expressions in the basal forebrain, hippocampus and cerebellum (P<0.05). The positivity rates of P-STAT3 in the two groups were 0.00%and 0.00%in the cerebral cortex, 68.75%and 0.00% in the basal forebrain, 62.50% and 12.50% in the hippocampus, and 43.75% and 0.00% in the cerebellum, respectively, showing also significant differences in the basal forebrain, hippocampus and cerebellum (P<0.05). The expression of STAT3 was positively correlated with that of P-STAT3 in transgenic AD mice (P<0.05). Conclusion STAT3 and P-STAT3 are highly expressed in the basal forebrain, hippocampus and cerebellum in transgenic AD mice and may participate in the pathological process of AD.