CAJ | 학술논문

目的：探讨低密度脂蛋白受体（low density lipoprotein receptor，LDL-R）基因单核苷酸多态性rs5932位点在人群中的分布及其与原发性高血压的关系。方法用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）技术分析132例健康对照人群和110例原发性高血压患者的LDL-R基因型及等位基因频率，并测定血脂水平。结果原发性高血压组和健康对照组的LDL-R基因型频率及等位基因频率的分布无统计学意义（χ2=0.180，P=0.962；χ2=0.101，P=0.766）；原发性高血压组中AA型的血浆LDL-C水平低于非AA型，（t=35.5，P<0.05）有统计学意义；健康对照组A等位基因频率高于高LDL-C组（χ2=4.321，P=0.04）。结论 LDL-R基因多态性可能与原发性高血压无相关性， A等位基因可能具有降低苗族人群血清LDL-C水平的作用。
목적：탐토저밀도지단백수체（low density lipoprotein receptor，LDL-R）기인단핵감산다태성rs5932위점재인군중적분포급기여원발성고혈압적관계。방법용취합매련반응-한제성편단장도다태성（PCR-RFLP）기술분석132례건강대조인군화110례원발성고혈압환자적LDL-R기인형급등위기인빈솔，병측정혈지수평。결과원발성고혈압조화건강대조조적LDL-R기인형빈솔급등위기인빈솔적분포무통계학의의（χ2=0.180，P=0.962；χ2=0.101，P=0.766）；원발성고혈압조중AA형적혈장LDL-C수평저우비AA형，（t=35.5，P<0.05）유통계학의의；건강대조조A등위기인빈솔고우고LDL-C조（χ2=4.321，P=0.04）。결론 LDL-R기인다태성가능여원발성고혈압무상관성， A등위기인가능구유강저묘족인군혈청LDL-C수평적작용。
Objective To investigate the relationship between essential hypertension and the single nucleotide polymorphisms(SNPs) of rs5932 of LDL-R gene in Xiangxi miao minority, in Hunan province. Methods 110 essential hypertension patients and 132 normal controls were recruited in the present study. The rs5932 polymorphism of LDL-R gene was analyzed by PCR-RFLP and the serum lipids were measured by automatic biochemical analyzer. Results There were three kinds of genotype:AA, AG and GG. No significant difference was found in genotype and allele frequencies of LDL-R gene polymorphism between essential hypertension group and the control group (χ2=0.180, P=0.962;χ2=0.101, P=0.766). The serum LDL-C levels of AA genotype was lower than that of non-AA genotype in essential hypertension group(t=35.5, P<0.05). Conclusion The A allele of rs5932 of LDL-R gene may be protective against the high level of LDL-C in miao minority. There was no association between LDL-R gene rs5932 polymorphism and EH in miao minority.