CAJ | 학술논문

目的：建立人血浆中盐酸美沙酮的HPLC分析方法。方法采用液-液萃取方法将人血浆中的盐酸美沙酮分离出来，利用C18柱，乙腈-0.02mol/L的磷酸二氢钾溶液（50∶50)，2mol/L的氢氧化钠调pH至5.5为流动相，检测波长220nm的色谱条件测定人血浆中盐酸美沙酮。结果盐酸美沙酮在1~100μg/mL的范围内与其峰面积线性关系良好（r=0.9743）。结论本法所建HPLC法操作简便，重复性好，可作为人血浆中盐酸美沙酮的分析测定方法。
목적：건립인혈장중염산미사동적HPLC분석방법。방법채용액-액췌취방법장인혈장중적염산미사동분리출래，이용C18주，을정-0.02mol/L적린산이경갑용액（50∶50)，2mol/L적경양화납조pH지5.5위류동상，검측파장220nm적색보조건측정인혈장중염산미사동。결과염산미사동재1~100μg/mL적범위내여기봉면적선성관계량호（r=0.9743）。결론본법소건HPLC법조작간편，중복성호，가작위인혈장중염산미사동적분석측정방법。
Objective To establish the method to determine the content of methadone in the human plasma. Methods Liquid-liquid extraction method is used to seperate methadone from human plasma, using column stuffed with C18, the mobile phase is mixed with acetonitrile of 0.02mol/L and potassium dihydrogen phosphate solution 2mol/L of phosphoric acid or sodium hydroxide of 2mol/L regulated pH to 5.5, the detection walvelength is 220 nm, apply above chromatographic condition to determine the content of methadone. Result Methadone hydrochloride have good linear relationship between peak area in 1-100μg/mL(r=0.9743). Conclusion The HPLC method is simple, and have good repeatability. It can be used as the content determination of methadone in human plasma.