CAJ | 학술논문

目的：研究盐酸埃克替尼联合放疗抗肿瘤的效果及机制。方法建立裸鼠人大肠腺癌HT29细胞株荷瘤模型，随机分为4组：对照组、盐酸埃克替尼组、放疗组、联合治疗组，用免疫荧光方法检测肿瘤组织γ-H2AX、53BP1表达，及细胞增殖相关抗原Ki-67和凋亡相关抗原Caspase-3的表达，并计算组织细胞内γ-H2AX、53BP1荧光焦点平均值以及肿瘤增殖率和肿瘤凋亡数。结果盐酸埃克替尼联合放疗组较其他3组显著增加肿瘤组织γ-H2AX和53BP1的表达，抑制肿瘤细胞增殖（Ki-67阳性细胞率降低）和增加肿瘤细胞凋亡（Caspase-3阳性细胞数增加）（均P＜0.01）。结论盐酸埃克替尼与放疗同步显著加重肿瘤细胞内的DNA损伤，抑制肿瘤增殖，增加肿瘤凋亡，从而增进放疗疗效。
목적：연구염산애극체니연합방료항종류적효과급궤제。방법건립라서인대장선암HT29세포주하류모형，수궤분위4조：대조조、염산애극체니조、방료조、연합치료조，용면역형광방법검측종류조직γ-H2AX、53BP1표체，급세포증식상관항원Ki-67화조망상관항원Caspase-3적표체，병계산조직세포내γ-H2AX、53BP1형광초점평균치이급종류증식솔화종류조망수。결과염산애극체니연합방료조교기타3조현저증가종류조직γ-H2AX화53BP1적표체，억제종류세포증식（Ki-67양성세포솔강저）화증가종류세포조망（Caspase-3양성세포수증가）（균P＜0.01）。결론염산애극체니여방료동보현저가중종류세포내적DNA손상，억제종류증식，증가종류조망，종이증진방료료효。
Objective To examine the antitumor efficacy of a combination of icotinib hydrochloride and radiotherapy in a human tumor xenograft model.Methods Tumor-bearing nude mouse models were established by using HT29 cells (a human colorectal adenocarcinoma cell line ).Four groups were set up in terms of different treatments (administration of icotinib hydro-chloride or irradiation ):control group ,icotinib hydrochloride group ;radiotherapy (RT ) group;icotinib hydrochloride + RT group.The expressions of γ-H2AX ,53BP1 Ki-67 and Caspase-3 were detected by immunofluorescence.The average fluorescent values of γ-H2AX and 53BP1 were obtained ,and the proliferation rate of tumor cells and the number of apoptotic cells calculat-ed.Results Icotinib hydrochloride combined with RT significantly inhibited the DNA repair by increasing the expression of γ-H2AX and 53BP1 and the treatment could inhibit the proliferation and increase the apoptosis of tumor tissue ( P< 0.01 ) . Conclusion Icotinib hydrochloride combined with RT enhances the radiosensitivity of tumor cells by aggravating the DNA inju-ry ,inhibiting the proliferation and increasing apoptosis of tumor cells.