广州化工
廣州化工
엄주화공
GUANGZHOU CHEMICAL INDUSTRY AND TECHNOLOGY
2012年
12期
142-143
,共2页
HPLC法%黄芪%毛蕊异黄酮葡萄糖苷
HPLC法%黃芪%毛蕊異黃酮葡萄糖苷
HPLC법%황기%모예이황동포도당감
HPLC%calycosin-7-O18-D-glycosid%radix astragalus
采用HPLC法测定了黄芪药材中毛蕊异黄酮葡萄糖苷的含量。以FL2200-II为高效液相色谱仪,采用Ultimate XB-C18色谱柱,流动相乙腈-0.2%甲酸溶液（20∶80）,检测波长260 nm,流速1.0 mL/min。毛蕊异黄酮葡萄糖苷在0.5～50μg/mL内线性良好,R2=0.9999;测得某黄芪药材中毛蕊异黄酮葡萄糖苷平均含量为31.86%,平均加标回收率99.55%,RSD为0.98%。利用反相高效液相色谱对黄芪药材中毛蕊异黄酮葡萄糖苷进行含量测定,方法简便,准确度高,重现性好,可应用于毛蕊异黄酮葡萄糖苷的含量测定。
採用HPLC法測定瞭黃芪藥材中毛蕊異黃酮葡萄糖苷的含量。以FL2200-II為高效液相色譜儀,採用Ultimate XB-C18色譜柱,流動相乙腈-0.2%甲痠溶液（20∶80）,檢測波長260 nm,流速1.0 mL/min。毛蕊異黃酮葡萄糖苷在0.5～50μg/mL內線性良好,R2=0.9999;測得某黃芪藥材中毛蕊異黃酮葡萄糖苷平均含量為31.86%,平均加標迴收率99.55%,RSD為0.98%。利用反相高效液相色譜對黃芪藥材中毛蕊異黃酮葡萄糖苷進行含量測定,方法簡便,準確度高,重現性好,可應用于毛蕊異黃酮葡萄糖苷的含量測定。
채용HPLC법측정료황기약재중모예이황동포도당감적함량。이FL2200-II위고효액상색보의,채용Ultimate XB-C18색보주,류동상을정-0.2%갑산용액（20∶80）,검측파장260 nm,류속1.0 mL/min。모예이황동포도당감재0.5～50μg/mL내선성량호,R2=0.9999;측득모황기약재중모예이황동포도당감평균함량위31.86%,평균가표회수솔99.55%,RSD위0.98%。이용반상고효액상색보대황기약재중모예이황동포도당감진행함량측정,방법간편,준학도고,중현성호,가응용우모예이황동포도당감적함량측정。
The content of calycosin-7-O18-D-glycosid in radix astragalus was determined with high performance liquid chromatography（HPLC）.Acted Ultimate XB-C18 as column,mobile phase was acetonitrile-water（0.2% Formic acid）=20：80,detection wavelength was 260 nm,and flow rate was 1.0 mL/min with FL2200-II HPLC.There was a good linear relationship when the concentration of Calycosin-7-O18-D-glycosid was in the range of 0.5~50 μg/mL（R2=0.9999）.The average content of calycosin-7-O18-D-glycosid in radix astragalus was 31.86%.The average recovery rate was 99.55%,and the RSD was 0.98%.The method was simple and easy and the repeatability was also very good,which can be used to determinate the content of calycosin-7-O18-D-glycosid.
