CAJ | 학술논문

本文利用组织切片的方法研究了双单倍体牙鲆的性腺发生、性别分化及早期发育。结果显示：双单倍体牙鲆中除雌性外，还有雄性和未分化鱼出现，所占比例分别为56%、38%和6%。双单倍体牙鲆雌性的性别分化晚于普通牙鲆。雌鱼至100日龄，卵巢腔仍未闭合；110日龄雌鱼的产卵板上存在大量向卵母过渡的卵原细胞，未见Ⅰ时相卵母细胞出现。双单倍体牙鲆雄鱼精巢的分化在时间上和普通牙鲆的基本相同。随着发育的进行，双单倍体牙鲆雌鱼的卵巢出现部分异常的卵母细胞以及卵巢退化现象；部分雄鱼的精巢也有退化现象出现。9~11月龄，双单倍体牙鲆雄鱼的精巢发育到Ⅲ期，而普通牙鲆性腺在同期进入了Ⅳ期；11月龄开始双单倍体牙鲆雄鱼精巢有退化的迹象；13~15月龄，精巢退化成精小叶，其中只含有精原细胞，初级精母细胞消失不见，似Ⅱ期精巢。
본문이용조직절편적방법연구료쌍단배체아평적성선발생、성별분화급조기발육。결과현시：쌍단배체아평중제자성외，환유웅성화미분화어출현，소점비례분별위56%、38%화6%。쌍단배체아평자성적성별분화만우보통아평。자어지100일령，란소강잉미폐합；110일령자어적산란판상존재대량향란모과도적란원세포，미견Ⅰ시상란모세포출현。쌍단배체아평웅어정소적분화재시간상화보통아평적기본상동。수착발육적진행，쌍단배체아평자어적란소출현부분이상적란모세포이급란소퇴화현상；부분웅어적정소야유퇴화현상출현。9~11월령，쌍단배체아평웅어적정소발육도Ⅲ기，이보통아평성선재동기진입료Ⅳ기；11월령개시쌍단배체아평웅어정소유퇴화적적상；13~15월령，정소퇴화성정소협，기중지함유정원세포，초급정모세포소실불견，사Ⅱ기정소。
In this paper,the gonadogenesis,gender differentiation and early stage develop-ment of doubled haploid Paralichthys olivaceus were studied by using histological sections. The results indicated that there were some females,males and also undifferentiated individuals in the doubled haploid population,and the ratio was 56%,38%and 6%,respectively. The gen-der differentiation in female doubled haploid was later than the normal one. For the female dou-ble haploid,at 100 days old,the ovarian cavity was still not closed;and at 110 days old,there were a large number of transitional cells from oogonium to oocyte on the spawning board,and the stageⅠoocyte was not observed. For the male double haploid,the gender differentiation was basically the same in time with the normal one. With the development of gonad,there were some abnormal oocytes in the ovaries of female double haploids,and some ovaries degenerat-ed. There were also some degeneration appeared in the testes of male doubled haploids. At 9~11 months old,the testis of male doubled haploid was at stageⅢ,while the testis of normal Japanese flounder developed into stage Ⅳ. From 11 months,the testes of some doubled hap-loids began to degenerate. At 13~15 months old,there were only spermatogonium in the semi-niferous lobules,and the primary spermatocyte disappeared,like the testis in stageⅡ.