CAJ | 학술논문

通过对连续两代减数分裂雌核发育牙鲆（Paralichthys olivaceus）再度诱导减数分裂雌核发育，首次获得三个连续三代减数分裂雌核发育二倍体家系（Meio-G3-1，Meio-G3-2，Meio-G3-3）。选用重组率高、中、低的30对微卫星引物，分析该三个家系的遗传构成。30个微卫星位点在三个家系中扩增到的等位基因数分别为35、38、37，平均等位基因数为1.17、1.27、1.23，平均观测杂合度（Ho）分别为0.1467、0.2556、0.2056，平均纯合度分别为0.8533、0.7444、0.7944。三个家系内个体间的平均遗传相似度为0.9913、0.9918、0.9838，母本与子代之间的平均遗传相似度为0.9946、0.9968、0.9923，三个家系间的遗传相似度分别为0.9717（Meio-G3-1和Meio-G3-2）、0.9810（Meio-G3-1和Meio-G3-3）、0.9714（Meio-G3-2和Meio-G3-3）。结果表明，连续三代诱导雌核发育能进一步提高个体的纯合度、个体间以及家系间的平均遗传相似度，是快速建立鱼类近交系的良好方法。
통과대련속량대감수분렬자핵발육아평（Paralichthys olivaceus）재도유도감수분렬자핵발육，수차획득삼개련속삼대감수분렬자핵발육이배체가계（Meio-G3-1，Meio-G3-2，Meio-G3-3）。선용중조솔고、중、저적30대미위성인물，분석해삼개가계적유전구성。30개미위성위점재삼개가계중확증도적등위기인수분별위35、38、37，평균등위기인수위1.17、1.27、1.23，평균관측잡합도（Ho）분별위0.1467、0.2556、0.2056，평균순합도분별위0.8533、0.7444、0.7944。삼개가계내개체간적평균유전상사도위0.9913、0.9918、0.9838，모본여자대지간적평균유전상사도위0.9946、0.9968、0.9923，삼개가계간적유전상사도분별위0.9717（Meio-G3-1화Meio-G3-2）、0.9810（Meio-G3-1화Meio-G3-3）、0.9714（Meio-G3-2화Meio-G3-3）。결과표명，련속삼대유도자핵발육능진일보제고개체적순합도、개체간이급가계간적평균유전상사도，시쾌속건립어류근교계적량호방법。
Three third-generation of successive meiogynogenetic families (Meio-G3-1,Meio-G3-2 and Meio-G3-3) in Japanese flounder (Paralichthys olivaceus) were induced by artificially meiogynogenesis,which the eggs were activated by UV-irradiated red sea bream (Pagrus ma-jor) sperm,and followed with the cold-shock treatment. We evaluated the genetic structure of these three families using 30 microsatellite markers,and the efficiency of successive meiogyno-genesis for producing highly inbred line was quantified by calculating the homozygosity and ge-netic similarity. The numbers of alleles detected were 35,38 and 37 in Meio-G3-1,Meio-G3-2 and Meio-G3-3,respectively. And for these three families studied,the average numbers of al-leles (A) were 1.17,1.27 and 1.23;the average observed heterozygosity (Ho) were 0.146 7, 0.255 6 and 0.205 6;the average homozygoisty among the 30 analyzed loci were 0.853 3, 0.744 4,and 0.794 4. For the genetic similarity,the average similarity index between offspring within family was 0.9913,0.991 8 and 0.983 8;between dam and offspring was 0.996,0.996 8 and 0.992 3;and between families was 0.971 7 (Meio-G3-1 and Meio-G3-2),0.981 0 (Meio-G3-1 and Meio-G3-3) and 0.971 4 (Meio-G3-2 and Meio-G3-3),respectively. Our results indi-cate that the artificially induced successive meiogynogenesis can increase the homozygosity of individual,and also the genetic similarity of offspring within family and between families. And therefore,the artificially induced successive meiogynogenesis is a good method to establish in-bred lines in fish.