CAJ | 학술논문

用甲醛灭活8种鲆鲽类主要病原菌，鳗弧菌(Vibrio anguillarum)、创伤弧菌(Vibrio vulnificus)、哈维弧菌(Vibrio harveyi)、溶藻弧菌(Vibrio alginolyticus)、嗜水气单胞菌(Aeromonas hydrophila)、豚鼠气单胞菌(Aeromonas caviae)、迟缓爱德华菌(Edwardsiella tarda)、鮰爱德华菌(Edwardsiella ictaluri)，制备成灭活疫苗后肌肉注射大菱鲆获得各病原菌抗血清。结果显示，抗鳗弧菌血清、抗哈维弧菌血清、抗豚鼠气单胞菌血清、抗鮰爱德华菌效价为1：6400，抗创伤弧菌血清、抗溶藻弧菌血清、抗嗜水气单胞菌血清效价为1：12800，抗迟缓爱德华菌血清效价为1：102400。应用ELISA分析各抗血清与8种病原菌及迟缓爱德华菌蛋白的免疫交叉反应，结果显示，各病原菌与其本身的抗血清反应最为强烈，而与其他抗血清间有程度不等的交叉反应；菌蛋白与其相应的菌抗血清反应最为强烈，与其他病原菌抗血清反应较弱。本研究所制备的病原菌抗血清效价较高，且具有特异性，可应用于鲆鲽类病原菌的检测。
용갑철멸활8충평접류주요병원균，만호균(Vibrio anguillarum)、창상호균(Vibrio vulnificus)、합유호균(Vibrio harveyi)、용조호균(Vibrio alginolyticus)、기수기단포균(Aeromonas hydrophila)、돈서기단포균(Aeromonas caviae)、지완애덕화균(Edwardsiella tarda)、회애덕화균(Edwardsiella ictaluri)，제비성멸활역묘후기육주사대릉평획득각병원균항혈청。결과현시，항만호균혈청、항합유호균혈청、항돈서기단포균혈청、항회애덕화균효개위1：6400，항창상호균혈청、항용조호균혈청、항기수기단포균혈청효개위1：12800，항지완애덕화균혈청효개위1：102400。응용ELISA분석각항혈청여8충병원균급지완애덕화균단백적면역교차반응，결과현시，각병원균여기본신적항혈청반응최위강렬，이여기타항혈청간유정도불등적교차반응；균단백여기상응적균항혈청반응최위강렬，여기타병원균항혈청반응교약。본연구소제비적병원균항혈청효개교고，차구유특이성，가응용우평접류병원균적검측。
In this study,formal dehyde was used to inactivate Edwardsiella tarda,Vibrio anguillarum, Vibrio harveyi, Vibrio vulnificus, Vibrio alginolyticus, Aeromonas caviae, Edwardsiella ictaluri,Aeromonas hydrophila,in order to preparethe inactivated vaccine. Then vaccine was used to immune turbot by intramuscular injection. In this way ,we got the antise-rum of all bacterium above. Enzyme-linked immunosorbent assay was used to determine the content of antibody in serum. The antibody can be detect after four week in the immune,and the level of antibody is research highest in the tenth week which is inoculated inactivated vac-cine of Edwardsiella tarda,and the highest titer of other vaccines are 1:102 400,1:6 400, 1:6 400,1:12 800,1:12 800,1:6400,1:6 400,1:12 800. The results of cross reaction of the bacteria and their anti-serum shows that the effect is most intense when the bacteria react with its own antiserum,and it is very weak when the bacteria react with other antiserum.