CAJ | 학술논문

目的：本研究旨在探讨低氧联合高脂饮食对SD大鼠心肌内皮型一氧化氮合酶（eNOS）/一氧化氮（NO）的影响及其可能机制。 　　方法：雄性SD大鼠60只随机分为三组，常氧组（南京，海拔10米）、低氧组（低压氧舱，模拟海拔5000米）、低氧联合高脂饮食组（低压氧舱，模拟海拔5000米），经普通饮食或高脂饮食4周后，留取外周血和心肌标本，用全自动血细胞分析仪检测静脉血血红蛋白浓度，TBA比色法检测血浆丙二醛含量，WST-1法检测超氧化物歧化酶活力，直接检测法和终点法检测血脂含量，荧光实时定量聚合酶链反应（PCR）检测心肌eNOS mRNA水平，蛋白印迹（Western blot）法检测心肌eNOS蛋白水平，硝酸还原酶法检测心肌NO代谢产物硝酸盐/亚硝酸盐（NOX）水平。 　　结果：低氧组及低氧联合高脂饮食组心肌eNOS mRNA及蛋白水平明显高于常氧组（P<0.05），低氧联合高脂饮食组心肌NOx水平明显低于其他两组（P<0.05）；与低氧组比较，低氧联合高脂饮食组血浆总胆固醇及低密度脂蛋白水平明显升高（P<0.05），血浆超氧化物歧化酶活力及丙二醛含量明显降低（P<0.05），差异有统计学意义。 　　结论：相对于单纯低氧，联合高脂饮食进一步降低大鼠心肌NOx水平，提示高脂饮食加重慢性重度低氧对心肌的损伤，其机制可能与血脂异常及抗氧化能力不足有关。
목적：본연구지재탐토저양연합고지음식대SD대서심기내피형일양화담합매（eNOS）/일양화담（NO）적영향급기가능궤제。 　　방법：웅성SD대서60지수궤분위삼조，상양조（남경，해발10미）、저양조（저압양창，모의해발5000미）、저양연합고지음식조（저압양창，모의해발5000미），경보통음식혹고지음식4주후，류취외주혈화심기표본，용전자동혈세포분석의검측정맥혈혈홍단백농도，TBA비색법검측혈장병이철함량，WST-1법검측초양화물기화매활력，직접검측법화종점법검측혈지함량，형광실시정량취합매련반응（PCR）검측심기eNOS mRNA수평，단백인적（Western blot）법검측심기eNOS단백수평，초산환원매법검측심기NO대사산물초산염/아초산염（NOX）수평。 　　결과：저양조급저양연합고지음식조심기eNOS mRNA급단백수평명현고우상양조（P<0.05），저양연합고지음식조심기NOx수평명현저우기타량조（P<0.05）；여저양조비교，저양연합고지음식조혈장총담고순급저밀도지단백수평명현승고（P<0.05），혈장초양화물기화매활력급병이철함량명현강저（P<0.05），차이유통계학의의。 　　결론：상대우단순저양，연합고지음식진일보강저대서심기NOx수평，제시고지음식가중만성중도저양대심기적손상，기궤제가능여혈지이상급항양화능력불족유관。
Objective: To investigate the effect of combined hypoxia and high fat diet (HFD) on endothelial nitric oxide synthase (eNOS)/nitric oxide (NO) of myocardium in experimental rats with its possible mechanisms. Methods: A total of 60 male SD rats were randomly divided into 3 groups, n=20 in each group. Control group, the rats were fed by normal diet with normal oxygen condition. Hypoxia group, the rats were fed by normal diet with simulated 5000m altitude oxygen condition. Combined hypoxia and HFD (H+HFD) group, the rats were fed by HFD and simulated 5000m altitude oxygen condition. All animals were treated for 4 weeks and peripheral blood and myocardium specimen were collected. Hemoglobin was examined by automatic blood cell analyzer, plasma malondialdehyde (MDA) was measured by TBA method, superoxide dismutase (SOD) activity was detected by WST-1 method, mRNA and protein expressions of eNOS were examined by real time PCR and Western blot analysis respectively, the myocardium nitrates and nitrites (NOx) was measured by nitrate reductase method. Results: Compared with Control group, Hypoxia group and H+HFD group had increased mRNA and protein expressions of eNOS, H+HFD group had lower NOx levels than the other 2 groups P<0.05. Compared with Hypoxia group, H+HFD group showed obviously increased total cholesterol, LDL-cholesterol and decreased SOD activity, diseased MDA level P<0.05. Conclusion: Upon hypoxia alone, H+HFD may further reduce NOx level of myocardium, it implies aggravated chronic hypoxia impairment, which might be related to dyslipidemia and lack of anti-oxidative ability in experimental rats.