CAJ | 학술논문

目的：分析鉴定短短芽胞杆菌Brevibacillus brevis的挥发性抑菌代谢物。方法采用二分隔平板测试短短芽胞杆菌FJAT-8672挥发性抑菌代谢物对尖孢镰刀菌的抑菌效果。运用顶空-固相微萃取-气质联用法对短短芽胞杆菌FJAT-8672的挥发性成分进行测定。结果短短芽胞杆菌FJAT-8672挥发性抑菌代谢物对尖孢镰刀菌的抑制率为36.10%。FJAT-8672菌落表面的中挥发性抑菌代谢物，共检测出高匹配度的挥发性抑菌代谢物21种。按照其化学结构不同分为醇类、醛类、酮类、酯类等。其中醛类化合物含量最高。结论发现短短芽胞杆菌挥发性抑菌代谢物的抑菌能力，其中主要生物活性代谢物包括两类，第一类可能具有抑菌抗癌等活性的代谢物，包括雪松醇、壬醛、癸醛；第二类可能与微生物代谢有关的代谢物，包括苯甲醛。
목적：분석감정단단아포간균Brevibacillus brevis적휘발성억균대사물。방법채용이분격평판측시단단아포간균FJAT-8672휘발성억균대사물대첨포렴도균적억균효과。운용정공-고상미췌취-기질련용법대단단아포간균FJAT-8672적휘발성성분진행측정。결과단단아포간균FJAT-8672휘발성억균대사물대첨포렴도균적억제솔위36.10%。FJAT-8672균락표면적중휘발성억균대사물，공검측출고필배도적휘발성억균대사물21충。안조기화학결구불동분위순류、철류、동류、지류등。기중철류화합물함량최고。결론발현단단아포간균휘발성억균대사물적억균능력，기중주요생물활성대사물포괄량류，제일류가능구유억균항암등활성적대사물，포괄설송순、임철、계철；제이류가능여미생물대사유관적대사물，포괄분갑철。
Objective To identify the antimicrobial volatile metabolites of Brevibacillus brevis FJAT-8672. Methods The antibacterial effect of the volatile metabolites from Brevibacillus brevis FJAT-8672 to Fusarium oxysporum was tested, by using the two separate plates. HS-SPME-GC/MS was applied to analyze the volatile metabolites of Brevibacillus brevis FJAT-8672. Results The inhibition rate of volatile metabolites from Brevibacillus brevis FJAT-8672 to Fusarium oxysporum was about 36.10% . Twenty-one volatile metabolites were identified with high compatibility. According to the chemical structure, they could be classified into alcohols, esters, aldehydes, ketones, and so on. Aldehyde compounds had the highest counts of the volatile metabolites. Conclusion There were two sorts of functional compounds in the FJAT-8672, one had the active ingredients such as antibacterial and anticancer, including Cedrol, Nonana and Decanal. The other related to the microbial metabolic metabolites, including Benzaldehyde.