CAJ | 학술논문

目的：研究该转基因羊奶表达的重组人溶菌酶的急性毒性和过敏性,确保食品质量安全。方法通过小鼠经口急性毒性、体外模拟胃肠道消化和体外热稳定性试验进行研究。结果小鼠经口急性毒性试验结果显示,重组人溶菌酶对小鼠最大耐受剂量＞5000 mg/kg.BW,属实际无毒。体外模拟胃肠道消化试验结果显示,重组人溶菌酶在模拟胃液中2~30 min内全部消化；在模拟肠液中15 s~2 min内全部消化；体外热稳定性试验结果显示,重组人溶菌酶在100℃加热60 min不会降解。结论该重组人溶菌酶的毒性与过敏性风险较低,可以安全应用于食品行业。
목적：연구해전기인양내표체적중조인용균매적급성독성화과민성,학보식품질량안전。방법통과소서경구급성독성、체외모의위장도소화화체외열은정성시험진행연구。결과소서경구급성독성시험결과현시,중조인용균매대소서최대내수제량＞5000 mg/kg.BW,속실제무독。체외모의위장도소화시험결과현시,중조인용균매재모의위액중2~30 min내전부소화；재모의장액중15 s~2 min내전부소화；체외열은정성시험결과현시,중조인용균매재100℃가열60 min불회강해。결론해중조인용균매적독성여과민성풍험교저,가이안전응용우식품행업。
Objective To ensure the food safety, this study was focused on the acute toxicity test and allergenicity assessment of lysozyme expressed in milk from transgenic goats andallergenicity assessment in vitro. Methods Oral acute toxicity test, thermal stability experiment and simulated gastrointestinal digestion experiment were put out to estimate the food safety of lysozyme. Results The oral acute toxicity test indicated the lysozyme protein caused no adverse effects in mice by intragastric infusion at a high dose of 5 g/kg.BW. The results of simulated gastrointestinal digestion experiment indicate that lysozyme was digested within 2~30 min in stimulated gastric fluid, and within 15 s~2 min in stimulated intestinal fluid. The thermal stability experiment showed that the lysozyme had stability after heating 60 min at 100℃. Conclusion The food safety evaluation of the lysozyme showed that it had littlerisk on allergenicity and toxicity, and it could be used in food industry safely.