CAJ | 학술논문

目的：优选沙棘果渣总黄酮提取的最佳工艺,并对其抗氧化活性进行评价。方法选取提取时间、温度、料液比、乙醇体积分数为影响因素,在单因素实验结果基础上确定各因素的分析水平,利用响应面中心组合法设计实验方案。并通过体外抗氧化能力指数(ORAC)法研究沙棘果渣总黄酮的抗氧化能力。结果建立了沙棘果渣总黄酮提取的数学模型,最佳提取工艺条件为乙醇体积分数70%、提取温度80℃、提取时间100 min、液料比25：1,沙棘果渣总黄酮具有较强抗氧化活性,与浓度成正相关。结论本研究结果可为沙棘果渣总黄酮工业化提取和应用提供理论指导。
목적：우선사극과사총황동제취적최가공예,병대기항양화활성진행평개。방법선취제취시간、온도、료액비、을순체적분수위영향인소,재단인소실험결과기출상학정각인소적분석수평,이용향응면중심조합법설계실험방안。병통과체외항양화능력지수(ORAC)법연구사극과사총황동적항양화능력。결과건립료사극과사총황동제취적수학모형,최가제취공예조건위을순체적분수70%、제취온도80℃、제취시간100 min、액료비25：1,사극과사총황동구유교강항양화활성,여농도성정상관。결론본연구결과가위사극과사총황동공업화제취화응용제공이론지도。
Objective To explore the optimal extraction conditions of total flavonoids from residue of Hippophae rhamnoides L. and evaluate the antioxidant activity. Methods Based on the single-factor tests, the conditions of total flavonoids from residue of Seabuckthorn were optimized by response surface methodology in order to increase the extraction yield of total flavonoids. Meanwhile, the antioxidant activity of total flavonoids was assessed by oxygen radical absorbent capacity (ORAC). Results The optimal extraction conditions of total flavonoids were ethanol concentration 70%, material-liquid ratio 1:25, extraction time 100 min and extraction temperature 80℃. Under the optimal conditions, the maximum extraction yield of total flavonoids was 2.73%. Extracted total flavonoids had a strong antioxidant activity in a dose-dependent manner. Conclusion These investigations will be reference a certain theoretical instruction and application value for the extraction of flavonoids in Seabuckthorn.