食品安全质量检测学报
食品安全質量檢測學報
식품안전질량검측학보
FOOD SAFETY AND QUALITY DETECTION TECHNOLOGY
2014年
9期
2792-2796
,共5页
连翘花%高效液相色谱法%芦丁%连翘酯苷A%连翘苷%松酯酚
連翹花%高效液相色譜法%蘆丁%連翹酯苷A%連翹苷%鬆酯酚
련교화%고효액상색보법%호정%련교지감A%련교감%송지분
Forsythia suspensa flower%high performance liquid chromatography%rutin%forsythoside A%forsythin%pinoresinol
目的：建立测定不同产地连翘花中芦丁、连翘酯苷 A、连翘苷、松酯酚含量的高效液相色谱法,为其有效成分控制提供有效方法。方法采用Symmetry C18(4.6 mm×150 mm,5μm)色谱柱,流动相为乙腈-水,梯度洗脱,流速1.0 mL/min,检测波长230 nm,柱温25℃。结果不同产地的连翘花之间各成分的含量差异显著,其中连翘酯苷A在各样品中含量最高,芦丁含量次之,连翘苷含量最少；其中菏泽产的样品连翘酯苷A含量高达119.38±5.78 mg/g,连翘苷的含量达4.71±0.09 mg/g；临沂产的样品芦丁含量最高达75.64±2.09 mg/g；日照、青岛和烟台的样品中松脂酚含量较高且相差不大,分别是25.41±1.51、24.45±0.98、23.60±1.19 mg/g。结论12个产地连翘花中连翘酯苷 A 的含量远远高于药典规定的最低含量要求。但连翘花中连翘苷的含量较低。
目的：建立測定不同產地連翹花中蘆丁、連翹酯苷 A、連翹苷、鬆酯酚含量的高效液相色譜法,為其有效成分控製提供有效方法。方法採用Symmetry C18(4.6 mm×150 mm,5μm)色譜柱,流動相為乙腈-水,梯度洗脫,流速1.0 mL/min,檢測波長230 nm,柱溫25℃。結果不同產地的連翹花之間各成分的含量差異顯著,其中連翹酯苷A在各樣品中含量最高,蘆丁含量次之,連翹苷含量最少；其中菏澤產的樣品連翹酯苷A含量高達119.38±5.78 mg/g,連翹苷的含量達4.71±0.09 mg/g；臨沂產的樣品蘆丁含量最高達75.64±2.09 mg/g；日照、青島和煙檯的樣品中鬆脂酚含量較高且相差不大,分彆是25.41±1.51、24.45±0.98、23.60±1.19 mg/g。結論12箇產地連翹花中連翹酯苷 A 的含量遠遠高于藥典規定的最低含量要求。但連翹花中連翹苷的含量較低。
목적：건립측정불동산지련교화중호정、련교지감 A、련교감、송지분함량적고효액상색보법,위기유효성분공제제공유효방법。방법채용Symmetry C18(4.6 mm×150 mm,5μm)색보주,류동상위을정-수,제도세탈,류속1.0 mL/min,검측파장230 nm,주온25℃。결과불동산지적련교화지간각성분적함량차이현저,기중련교지감A재각양품중함량최고,호정함량차지,련교감함량최소；기중하택산적양품련교지감A함량고체119.38±5.78 mg/g,련교감적함량체4.71±0.09 mg/g；림기산적양품호정함량최고체75.64±2.09 mg/g；일조、청도화연태적양품중송지분함량교고차상차불대,분별시25.41±1.51、24.45±0.98、23.60±1.19 mg/g。결론12개산지련교화중련교지감 A 적함량원원고우약전규정적최저함량요구。단련교화중련교감적함량교저。
Objective To establish a method for determination of rutin, forsythoside A, forsythin and pinoresinol in different regions of Forsythia suspensa flower by high performance liquid chromatography, thus to offer a method for its quality control. Methods Symmetry C18 (4.6 mm×150 mm, 5 μm) column was adopted. The mobile phase was acetonitrile-water with gradient elution at the flow rate of 1.0 mL/min. The detection wavelength was 230 nm, and the column temperature was 25 ℃. Results The contents of rutin, forsythoside A, forsythin and pinoresinol in different regions of F. suspensa flower were significantly different, and the contents of forsythoside A in the F. suspensa flower was the highest, then followed by rutin and forsythin. Among them, the content of forsythoside A and forsythin of the F. suspensa flower in region of Heze were 119.38±5.78 mg/g and 4.71±0.09 mg/g, respectively. The content of rutin of the F. suspensa flower in region of Linyi was 75.64±2.09 mg/g, which was the highest. High content of pinoresinol in regions of Rizhao, Qingdao and Yantai are 25.41±1.51, 24.45±0.98 and 23.60±1.19 mg/g, respectively, which showed no significant difference. Conclusion Although the content of forsythin of F. suspensa flower was low, the content of forsythoside A in 12 regions was significantly higher than that of the Chinese Pharmacopoeia requirement.
