CAJ | 학술논문

乳源抗氧化肽具有原料来源广泛、抗氧化活性强、天然无毒副作用等优点，已成为国内外学者的研究热点。为此，本文综述了乳源抗氧化肽的研究概况。首先探讨了乳源抗氧化肽的六大制备途径，发现酶解制备法使用最多。再总结了分离纯化的方法，一般为几种方法结合使用，层层递进的得到纯度较高的抗氧化肽。比较了多种抗氧化活性测定方法，在乳源抗氧化肽领域使用最多的是体外自由基清除率的测定方法，并总结了抗氧化肽分子特征，主要是由2~20个氨基酸组成的小肽，分子量在5000 Da以下，肽段中疏水性氨基酸和芳香族氨基酸占优势，但具体的构效关系还未明确。阐述了其在食品当中的应用研究现状，并展望了将来的研究及应用方向。乳源抗氧化肽显示出一定的优势，具有广阔的市场前景。
유원항양화태구유원료래원엄범、항양화활성강、천연무독부작용등우점，이성위국내외학자적연구열점。위차，본문종술료유원항양화태적연구개황。수선탐토료유원항양화태적륙대제비도경，발현매해제비법사용최다。재총결료분리순화적방법，일반위궤충방법결합사용，층층체진적득도순도교고적항양화태。비교료다충항양화활성측정방법，재유원항양화태영역사용최다적시체외자유기청제솔적측정방법，병총결료항양화태분자특정，주요시유2~20개안기산조성적소태，분자량재5000 Da이하，태단중소수성안기산화방향족안기산점우세，단구체적구효관계환미명학。천술료기재식품당중적응용연구현상，병전망료장래적연구급응용방향。유원항양화태현시출일정적우세，구유엄활적시장전경。
Milk-derived antioxidative peptides have many advantages with a wide range of sources, a strong antioxidant activity and non-toxic side effects, and it has become a hot research at home and abroad. In recent years, researchers have done a lot of work on the milk-derived antioxidative peptides. This paper mainly reviewed the research situation of the milk-derived antioxidative peptides. Six major preparation ways of milk-derived antioxidative peptides were discussed, it found that the enzymatic preparation method was used the most. Then the methods of separation and purification were summarized, usually researchers combined several methods to obtained high purity of peptides. And several antioxidation assay methods were compared, in the field of milk-derived antioxidative peptides, measuring scavenging rate of free radical in vitro was used most. Molecular characteristics of antioxidation peptides were summarized, they are composed of 2 to 20 amino acids, and their molecular weight were less than 5000Da. The hydrophobic amino acids and aromatic amino acids are dominant in the peptide fragment, but the specific structure-activity relationship is not clear. Their applications in food were expounded and the future directions of milk-derived antioxidant peptides were put forward. Milk-derived antioxidative peptides possessed superiority, and have widespread market prospect.