CAJ | 학술논문

目的：对酶联免疫吸附测定法(enzyme-linked immunosorbent assay, ELISA)检测莱克多巴胺假阳性结果产生的原因、影响因素和质量控制方法进行分析研究。方法应用经验证可靠的ELISA方法对进口猪肉产品进行莱克多巴胺兽药残留初筛检测,对阳性可疑样品采用液相色谱-串联质谱分析方法进行确证检测,并对两种方法的检测结果进行对比分析。结果样品在腐败变质后产生含有可以产生非特异性显色内源性辣根过氧化物酶的细菌,从而造成假阳性,干扰检测结果。结论莱克多巴胺检测中造成酶联免疫吸附(ELISA)方法假阳性结果的主要原因包括莱克多巴胺的代谢速度快, ELISA检测所用抗体与莱克多巴胺的交叉反应性高,以及样品因素的影响。
목적：대매련면역흡부측정법(enzyme-linked immunosorbent assay, ELISA)검측래극다파알가양성결과산생적원인、영향인소화질량공제방법진행분석연구。방법응용경험증가고적ELISA방법대진구저육산품진행래극다파알수약잔류초사검측,대양성가의양품채용액상색보-천련질보분석방법진행학증검측,병대량충방법적검측결과진행대비분석。결과양품재부패변질후산생함유가이산생비특이성현색내원성랄근과양화물매적세균,종이조성가양성,간우검측결과。결론래극다파알검측중조성매련면역흡부(ELISA)방법가양성결과적주요원인포괄래극다파알적대사속도쾌, ELISA검측소용항체여래극다파알적교차반응성고,이급양품인소적영향。
Objective To research the reasons, influence factors and quality control methods of the false-positive results for ractopamine detection by enzyme-linked immunosorbent assay (ELISA). Methods The ractopamine residues in imported porcine products was detected using ELISA, and positive samples were further determined by HPLC-MS/MS confirmatory method. Furthermore, the results of those two methods were compared and analyzed. Results The bacteria could thrive in the spoilage samples and gendogenous horseradish peroxidase contained in it. It caused nonspecific coloration resulting in false positive results and interference of the detection. Conclusion The reasons which cause the false positive by ELISA to detect ractopamine are the fast metabolic rate of ractopamine, the high cross reactivity of ractopamine antibody by ELISA and the effect of sample factors.