CAJ | 학술논문

目的：建立榛果过敏原成分的荧光定量PCR检测方法,并将此方法与酶联免疫吸附法(enzyme-linked immunosorbent assay, ELISA)方法进行比对实验。方法根据榛果成分oleosin特异性基因设计并筛选合适的引物和探针,优化反应体系和反应条件,建立榛果过敏原成分的荧光定量 PCR 检测方法,对荧光定量 PCR 方法与ELISA方法检测结果进行分析。结果建立的榛果过敏原成分荧光定量PCR方法特异性良好,可用于榛果过敏原成分的定量检测,但检测灵敏度低于ELISA检测方法。结论所建立的榛果过敏原成分的荧光定量PCR检测方法特异性好,灵敏度达到10 mg/kg,具有较好的实用性, ELISA检测方法灵敏度高于荧光定量PCR法,但当榛果过敏蛋白被破坏后有可能出现假阴性结果。
목적：건립진과과민원성분적형광정량PCR검측방법,병장차방법여매련면역흡부법(enzyme-linked immunosorbent assay, ELISA)방법진행비대실험。방법근거진과성분oleosin특이성기인설계병사선합괄적인물화탐침,우화반응체계화반응조건,건립진과과민원성분적형광정량 PCR 검측방법,대형광정량 PCR 방법여ELISA방법검측결과진행분석。결과건립적진과과민원성분형광정량PCR방법특이성량호,가용우진과과민원성분적정량검측,단검측령민도저우ELISA검측방법。결론소건립적진과과민원성분적형광정량PCR검측방법특이성호,령민도체도10 mg/kg,구유교호적실용성, ELISA검측방법령민도고우형광정량PCR법,단당진과과민단백피파배후유가능출현가음성결과。
Objective To establish a real-time PCR method for detection of the allergen of hazelnut, and compare the method with the enzyme-linked immunosorbent assay (ELISA) method. Methods According to the oleosin gene of hazelnut, the optimized reaction system and the reaction conditions had been proposed. To establish a real-time PCR method for detection of the allergen of hazelnut, a comparative analysis of the detection results by this method and ELISA was made. Results The method was specific for hazelnut allergens, and was suitable for the detection of hazelnut allergens. But the sensitivity of this method was lower than ELISA. Conclusion The real-time PCR method for detecting the allergen of hazelnut is suitable for application, the sensitivity is up to 10 mg/kg, lower than the ELISA detection method, but a false negative result may occur in ELISA method due to the hazelnut allergic protein damaged.