CAJ | 학술논문

近几年来由于一些企业为降低生产成本，常常会在乳与乳制品中掺假，这种行为严重影响了乳品加工企业的产品质量，同时也对消费者的身体健康造成了严重损害。在各种乳与乳制品掺假方法中，添加蛋白类掺假物如水解蛋白是比较常见的方法。L-羟脯氨酸是水解蛋白特有的氨基酸，而乳蛋白质中是不含L-羟脯氨酸的，因此检测乳与乳制品中是否含有L-羟脯氨酸是判断乳与乳制品是否掺假的一个重要手段之一。目前，针对L-羟脯氨酸的检测方法主要有分光光度法、液相色谱法、氨基酸分析仪法、液相色谱质谱法等。本文综述了近几年来国内外乳与乳制品中 L-羟脯氨酸的检测技术及其研究进展，对上述几种检测方法各自的优缺点进行了详细的分析，同时对未来的L-羟脯氨酸检测技术发展进行了展望。
근궤년래유우일사기업위강저생산성본，상상회재유여유제품중참가，저충행위엄중영향료유품가공기업적산품질량，동시야대소비자적신체건강조성료엄중손해。재각충유여유제품참가방법중，첨가단백류참가물여수해단백시비교상견적방법。L-간포안산시수해단백특유적안기산，이유단백질중시불함L-간포안산적，인차검측유여유제품중시부함유L-간포안산시판단유여유제품시부참가적일개중요수단지일。목전，침대L-간포안산적검측방법주요유분광광도법、액상색보법、안기산분석의법、액상색보질보법등。본문종술료근궤년래국내외유여유제품중 L-간포안산적검측기술급기연구진전，대상술궤충검측방법각자적우결점진행료상세적분석，동시대미래적L-간포안산검측기술발전진행료전망。
In recent years, some enterprises have made adulteration in milk and dairy products to reduce production cost. This kind of behavior seriously affect the quality of dairy products, and also caused serious damage to health of consumers. Among various kinds of adulterated milk and dairy products method, the common method is adding protein adulteration objects such as protein hydrolysate. L-hydroxyproline is a hydrolyzed protein, but milk protein does not include L-hydroxyproline. Detection of L-hydroxyproline is one of important methods to identify whether the addition of animal protein in milk and dairy products. In this paper, several methods to detect the L-hydroxyproline, including spectrophotometry, high performance liquid chromatography, automatic amino acid analyzer and high performance liquid chromatography-mass spectrometry were reviewed. In addition, the trends of related methods to detect L-hydroxyproline in milk and dairy products were predicted.