CAJ | 학술논문

目的：建立调味面制品中富马酸二甲酯快速、灵敏的高效液相色谱测定方法。方法色谱柱为ODS-C184.6 mm×250 mm,流动相为甲醇+(0.02 mol/L)乙酸铵(55：45, v：v)。样品粉碎用氨水甲醇溶液超声提取后,用盐酸调节pH为6,经0.45μm滤膜过滤,进样测定,保留时间定性,峰面积定量。结果标准曲线线性良好,方法线性范围0.25~10 mg/L,线性相关系数为0.9995,检出限为0.03 mg/kg,定量限为0.1 mg/kg,加标回收率在94.0%~102.5%范围内,相对标准偏差在3.6%~5.2%之间。结论该方法操作简便、具有灵敏高、准确性好、无杂质干扰等优点,可用于调味面制品中富马酸二甲酯的测定。
목적：건립조미면제품중부마산이갑지쾌속、령민적고효액상색보측정방법。방법색보주위ODS-C184.6 mm×250 mm,류동상위갑순+(0.02 mol/L)을산안(55：45, v：v)。양품분쇄용안수갑순용액초성제취후,용염산조절pH위6,경0.45μm려막과려,진양측정,보류시간정성,봉면적정량。결과표준곡선선성량호,방법선성범위0.25~10 mg/L,선성상관계수위0.9995,검출한위0.03 mg/kg,정량한위0.1 mg/kg,가표회수솔재94.0%~102.5%범위내,상대표준편차재3.6%~5.2%지간。결론해방법조작간편、구유령민고、준학성호、무잡질간우등우점,가용우조미면제품중부마산이갑지적측정。
Objective To establish a rapid and sensitive high performance liquid chromatography method for determination of demethyl fumarate in seasoned flour products. Methods Chromatographic column was ODS-C18 4.6 mm×250 mm. The mobile phase was methanol 0.02 mol/L ammonium acetate (55:45, v:v). Sample was crushed and extracted with ammonia water methanol solution; pH value was adjusted to 6 with hydrochloric acid;0.45μm membrane was used for filtration after ultrasonic extraction. Then, the sample was injected into high performance liquid chromatography, the component was qualitatively determined by retention time and quantitatively determined by peak area. Results Under the optimal conditions, the method showed a good linearity in the range of 0.25~10.0 mg/L, and the correlation coefficient was 0.9995. The limit of detection was 0.03 mg/kg, and the limit of quantification was 0.1 mg/kg. The recovery percentages varied from 94.0% to 102.5% and relative standard deviations were in the range 3.6%~5.2%. Conclusion The method was proved to be satisfactory in precision, accuracy and sensitivity, and without the distraction of impurities. It can be used to detect demethyl fumarate in seasoned flour products.