临床口腔医学杂志
臨床口腔醫學雜誌
림상구강의학잡지
JOURNAL OF CLINICAL STOMATOLOGY
2014年
9期
519-521
,共3页
耿海霞%封伟%钱君荣%郭秀娟
耿海霞%封偉%錢君榮%郭秀娟
경해하%봉위%전군영%곽수연
羟基磷灰石/凝胶支架%成骨细胞%组织学%骨缺损
羥基燐灰石/凝膠支架%成骨細胞%組織學%骨缺損
간기린회석/응효지가%성골세포%조직학%골결손
hydroxyapatite/geltin scaffold%osteoblasts%Histology%Bone defect
目的:通过组织学研究评价羟基磷灰石/凝胶(HAP-GEL)支架复合成骨细胞在兔颅骨缺损中的修复效果。方法:将HAP-GEL复合物与成骨细胞联合培养,制备兔颅顶骨骨缺损模型,并设置空白对照。于术后4,8,12周通过组织学观察分析骨缺损修复情况。结果:组织学观察,随着时间的推移,HAP-GEL支架组复合成骨细胞逐步出现降解并有新生骨小梁产生,材料密度降低。结论:HAP-GEL支架复合成骨细胞在兔颅骨缺损中可取得较好的修复效果。
目的:通過組織學研究評價羥基燐灰石/凝膠(HAP-GEL)支架複閤成骨細胞在兔顱骨缺損中的脩複效果。方法:將HAP-GEL複閤物與成骨細胞聯閤培養,製備兔顱頂骨骨缺損模型,併設置空白對照。于術後4,8,12週通過組織學觀察分析骨缺損脩複情況。結果:組織學觀察,隨著時間的推移,HAP-GEL支架組複閤成骨細胞逐步齣現降解併有新生骨小樑產生,材料密度降低。結論:HAP-GEL支架複閤成骨細胞在兔顱骨缺損中可取得較好的脩複效果。
목적:통과조직학연구평개간기린회석/응효(HAP-GEL)지가복합성골세포재토로골결손중적수복효과。방법:장HAP-GEL복합물여성골세포연합배양,제비토로정골골결손모형,병설치공백대조。우술후4,8,12주통과조직학관찰분석골결손수복정황。결과:조직학관찰,수착시간적추이,HAP-GEL지가조복합성골세포축보출현강해병유신생골소량산생,재료밀도강저。결론:HAP-GEL지가복합성골세포재토로골결손중가취득교호적수복효과。
Objective:To investigate the ability of HAP-GEL/osteoblasts scaffolds to repair cranial bone defect by his-tological study. Method:HAP-GEL and osteoblasts were co-culture.The rabbits cranial parietal bone defect models were prepared.HAP-GEL scaffolds and osteoblasts,HAP-GEL scaffolds and blank defect were implanted into the cranial bone de-fects area respectively. After 4,8,12 weeks,the results were evaluated by histological examination. Result:Histological ob-servation showed HAP-GEL/osteoblasts materials had appeared degradation and the formation of new trabecular bone ,the density of the material decreased gradualy. Conclusion:The engineering bone scaffold constructed by HAP-GEL scaffolds and osteoblasts can be applied to the repair of bone defect.