CAJ | 학술논문

目的：观察上皮标志物和转录因子之间的相关性，从而确定这些因子在肿瘤上皮-间质转化（EMT）过程中所起的作用，进一步研究其与口腔鳞癌临床病理之间的关系，揭示其在口腔鳞癌发生发展中的作用及临床意义。方法：免疫组化SP法检测57例口腔鳞癌组织和10例正常口腔黏膜组织中E-cad、Snail、Slug、MMP-2、MMP-9表达，分析其与口腔鳞癌临床病理的关系及E-cad和Snail、Slug、MMP-2、MMP-9表达的相关性。结果：①．口腔鳞癌中E-cad、Snail、Slug、MMP-2、MMP-9的阳性表达率分别为29．82％、75．43％、82．46％、71．93％、78．95％。②．口腔鳞癌中E-cad的表达与分化程度有关（P＜0．05），Snail、Slug、MMP-2、MMP-9的表达与分化程度无关（P＞0．05）；③口腔鳞癌中E-cad、Slug、MMP-2、MMP-9的表达与浸润和淋巴结转移有关（P＜0．05），Snail与淋巴结转移和浸润无关（P＞0．05）。④．口腔鳞癌中E-cad的表达与Snail、Slug、MMP-9表达均呈负相关（P＜0．05），而与MMP-2的表达无关（P＞0．05）。结论：转录因子与上皮标志物之间相关性明显，它们与口腔鳞癌的侵袭、淋巴结转移和分化程度相关。这些因子在EMT过程中不同阶段起作用，它们之间的相互作用是口腔鳞癌发生发展的重要环节。
목적：관찰상피표지물화전록인자지간적상관성，종이학정저사인자재종류상피-간질전화（EMT）과정중소기적작용，진일보연구기여구강린암림상병리지간적관계，게시기재구강린암발생발전중적작용급림상의의。방법：면역조화SP법검측57례구강린암조직화10례정상구강점막조직중E-cad、Snail、Slug、MMP-2、MMP-9표체，분석기여구강린암림상병리적관계급E-cad화Snail、Slug、MMP-2、MMP-9표체적상관성。결과：①．구강린암중E-cad、Snail、Slug、MMP-2、MMP-9적양성표체솔분별위29．82％、75．43％、82．46％、71．93％、78．95％。②．구강린암중E-cad적표체여분화정도유관（P＜0．05），Snail、Slug、MMP-2、MMP-9적표체여분화정도무관（P＞0．05）；③구강린암중E-cad、Slug、MMP-2、MMP-9적표체여침윤화림파결전이유관（P＜0．05），Snail여림파결전이화침윤무관（P＞0．05）。④．구강린암중E-cad적표체여Snail、Slug、MMP-9표체균정부상관（P＜0．05），이여MMP-2적표체무관（P＞0．05）。결론：전록인자여상피표지물지간상관성명현，타문여구강린암적침습、림파결전이화분화정도상관。저사인자재EMT과정중불동계단기작용，타문지간적상호작용시구강린암발생발전적중요배절。
Objective:To study the relationship of epithelial markers and transcription factors and confirm their func-tions in OSCC EMT and investigate their relationship between these factors and clinicopathologic features of OSCC in order to further reveal their roles during OSCC progression. Method:SP immunohistochemical method was used to detect the ex-pression of E-cad,Snail,Slug,MMP-2 and MMP-9 expression in 57 cases of OSCC and 10 cases of normal oral mucosa,an-alyze their relationship with clinicopathological factors of OSCC and correlation between E-cad and Snail,Slug,MMP-2, MMP-9 expression. Result: ①.In OSCC tissues,the positive rate of E-cad,Snail,Slug,MMP-2,MMP-9 respectively were 29.82%,75.43%,82.46%,71.93%,78.95%.2 (1) Expression of E-cad was related to differentiation degree of OSCC(P<0.05),but Snail,Slug,MMP-2,MMP-9 were not(P>0.05);②Expression of E-cad,Slug,MMP-2,MMP-9 had significantly positively correlated with invasion and lymph node metastasis(P<0.05),Snail has nothing to do with the invasion and lymph node metastasis(P>0.05). 3 The expression between E-cad and Snail,Slug,MMP-9 expression was negatively correlated in OSCC(P <0.05),but not related to MMP-2(P >0.05). Conclusion:Transcription factors and epithelial markers were corre-lated significantly,they were related to the invasion and lymph node metastasis and differentiation of oral squamous cell car-cinoma. These factors play a role in the different stages of EMT process, the interaction of them is an important part of the development of OSCC.