中国林副特产
中國林副特產
중국림부특산
QUARTERLY OF FOREST BY-PRODUCT AND SPECIALITY IN CHINA
2014年
2期
25-26
,共2页
丹东野百合%组织培养%快速繁殖
丹東野百閤%組織培養%快速繁殖
단동야백합%조직배양%쾌속번식
Lillum brownii%Tissure culture%Rapid propagation
对丹东地区野生香百合的组织培养进行了研究。结果表明：以球根鳞片做外植体培养在MS＋BA 1．0 mg/L ＋ NAA 0．4 mg/L培养基上可诱导出小鳞茎，在MS＋BA 1 mg/L ＋ NAA 0．1 mg/L培养基上继代可增殖，增殖系数5倍以上；壮苗培养以MS＋BA 0．5 mg/L ＋ NAA 0．1 mg/L 为好；最适生根培养为1/2M S＋IBA 0．5 mg/L ，生根率达90％，；移栽基质以纯沙为最好，成活率达95％以上。
對丹東地區野生香百閤的組織培養進行瞭研究。結果錶明：以毬根鱗片做外植體培養在MS＋BA 1．0 mg/L ＋ NAA 0．4 mg/L培養基上可誘導齣小鱗莖，在MS＋BA 1 mg/L ＋ NAA 0．1 mg/L培養基上繼代可增殖，增殖繫數5倍以上；壯苗培養以MS＋BA 0．5 mg/L ＋ NAA 0．1 mg/L 為好；最適生根培養為1/2M S＋IBA 0．5 mg/L ，生根率達90％，；移栽基質以純沙為最好，成活率達95％以上。
대단동지구야생향백합적조직배양진행료연구。결과표명：이구근린편주외식체배양재MS＋BA 1．0 mg/L ＋ NAA 0．4 mg/L배양기상가유도출소린경，재MS＋BA 1 mg/L ＋ NAA 0．1 mg/L배양기상계대가증식，증식계수5배이상；장묘배양이MS＋BA 0．5 mg/L ＋ NAA 0．1 mg/L 위호；최괄생근배양위1/2M S＋IBA 0．5 mg/L ，생근솔체90％，；이재기질이순사위최호，성활솔체95％이상。
The rapid propagation of Lillum brownii was developed through culture of scales and stem seg-ments in vitro .The best induction medium for scales was MS+BA 1 .0 mg/L + NAA 0 .4 mg/L ;the best proliferation medium for shoots was MS +BA 1 mg/L + NAA 0 .1 mg/L .Propagation coefficient was 5 times .MS+BA 0 .5 mg/L + NAA 0 .1 mg/L on seedling cultivation as well .The best induction medium for roots was 1/2MS+IBA0 .5 mg/L .the rate was 90% .When micropropagated plantlets with well-developed root systems were transferred to planting bed of sand in a greenhouse ,95% of the plant-lets survived .
