CAJ | 학술논문

基于酶催化阴极电致化学发光（ECL）构建灵敏的免疫传感器用于肿瘤标志物检测。利用过氧化氢酶（HRP）催化H2O2底物反应产生大量溶解氧，催化增强S2O82-的阴极ECL信号，构建非标记型免疫传感器。当抗原蛋白在电极表面与抗体形成免疫复合物时，屏蔽了检测液中底物与电极表面固载酶之间的接触并阻碍了电子传递，使ECL信号强度随抗原浓度增加而减小，建立ECL信号与待测物浓度之间的线性依赖关系，实现对血清中肿瘤标志物CEA的快速灵敏检测，检测限达1.04 ng/mL。
기우매최화음겁전치화학발광（ECL）구건령민적면역전감기용우종류표지물검측。이용과양화경매（HRP）최화H2O2저물반응산생대량용해양，최화증강S2O82-적음겁ECL신호，구건비표기형면역전감기。당항원단백재전겁표면여항체형성면역복합물시，병폐료검측액중저물여전겁표면고재매지간적접촉병조애료전자전체，사ECL신호강도수항원농도증가이감소，건립ECL신호여대측물농도지간적선성의뢰관계，실현대혈청중종류표지물CEA적쾌속령민검측，검측한체1.04 ng/mL。
A free-labeled ECL enzyme-linked immunoassay was successfully designed for the assay of CEA. This method employed the reaction between enzyme and substrate to generate amounts of dissolved oxygen for catalyzing the cathodic ECL signal of peroxydisulfate and constructing the label -free immunosensor. Based on the immuconjugated antigen on the electrode surface shielding the contact between the substrate and the solid-state mobilized enzyme, the ECL signal decreased with the increasing of analyte level. Thus, the label-free ECL immunosensor employed the enzyme to catalyze the cathodic ECL signal and built on the dependent relationship between ECL signal and the CEA antigen level. The detected limit for CEA antigen reached 1.04 ng/mL.