CAJ | 학술논문

目的：探讨Sam68在结直肠癌发生发展中的作用。方法建立Sam68稳定过表达和稳定干扰的结直肠癌细胞株，通过克隆形成实验、MTT法和Transwell侵袭实验检测Sam68对结直肠癌细胞增殖、侵袭和迁移能力的影响。结果成功建立Sam68稳定过表达和稳定干扰细胞株；过表达Sam68的结直肠癌细胞株SW480和Ls174t增殖、侵袭和迁移能力明显增强（P<0.05）；干扰Sam68的结直肠癌细胞株SW620和HCT116增殖、侵袭和迁移能力明显下降（P<0.05）。结论 Sam68促进结直肠癌细胞的增殖、侵袭和迁移能力，在结直肠癌发生发展过程中起重要作用。
목적：탐토Sam68재결직장암발생발전중적작용。방법건립Sam68은정과표체화은정간우적결직장암세포주，통과극륭형성실험、MTT법화Transwell침습실험검측Sam68대결직장암세포증식、침습화천이능력적영향。결과성공건립Sam68은정과표체화은정간우세포주；과표체Sam68적결직장암세포주SW480화Ls174t증식、침습화천이능력명현증강（P<0.05）；간우Sam68적결직장암세포주SW620화HCT116증식、침습화천이능력명현하강（P<0.05）。결론 Sam68촉진결직장암세포적증식、침습화천이능력，재결직장암발생발전과정중기중요작용。
Objective To investigate the role of Sam68 (Src-associated substrate during mitosis 68 kD) in the occurrence and development of colorectal cancer. Methods Colorectal cancer cell lines with stable Sam68 over-expressing and low Sam68 expression were established to test the effect of Sam68 in the proliferation, invasion, and migration of the cancer cells using colony formation, MTT and Transwell assays. Results SW480 and Ls174t colorectal cell lines over-expressing Sam68 showed significantly enhanced cell proliferation, invasion and migration (P<0.05). Conversely, the low Sam68 expression in SW620 and HCT116 colorectal cell lines significantly suppressed the cell proliferation, invasion and migration (P<0.05). Conclusion The expression of Sam68 can promote the proliferation, invasion and migration of colorectal cancer cells lines in vitro.